Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,582,677	G→A	T34I (ACC→ATC)	ypfG ←	DUF1176 domain‑containing protein YpfG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,582,677	0	G	A	100.0%	115.6 / NA	33	T34I (ACC→ATC)	ypfG	DUF1176 domain‑containing protein YpfG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/23);  total (10/23)

GCTGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCCACACCAAAGACGTCGGCAACAAAGCGAAAAA  >  NC_000913/2582601‑2582750                                                                             |                                                                          gcTGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGATGAc                                                                       >  1:460326/1‑81 (MQ=255)    gCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGATGACCTg                                                                    >  1:460329/1‑81 (MQ=255)     cGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGATGACCTGc                                                                   <  2:460269/81‑1 (MQ=255)      ggCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGATGACCTGcc                                                                  <  2:460270/81‑1 (MQ=255)          cAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGATGTCCTGCCAGTc                                                              >  1:460313/1‑81 (MQ=255)           aGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGATGACCTGCCAGt                                                               <  2:460271/79‑1 (MQ=255)              gTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGaa                                                          <  1:460272/81‑1 (MQ=255)                 atcaCCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGAAAAc                                                       <  1:460273/81‑1 (MQ=255)                 atcaCCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGAAAAc                                                       <  1:460274/81‑1 (MQ=255)                    aCCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGAAAACGcc                                                    >  1:460317/1‑81 (MQ=255)                       aGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGAAAACTCCCGt                                                 <  1:460276/81‑1 (MQ=255)                       aGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGAAAACGCCCGt                                                 <  1:460275/81‑1 (MQ=255)                       aGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGAAAACGCCCGt                                                 <  2:460277/81‑1 (MQ=255)                           cATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGAAAACGCCCGTTgcg                                             >  1:460332/1‑81 (MQ=255)                               aTGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGAAAACGCCCGTTGCGCTgg                                         >  1:460335/1‑81 (MQ=255)                                 gATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTg                                       >  1:460334/1‑81 (MQ=255)                                  aTCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTgc                                      >  2:460331/1‑81 (MQ=255)                                     gCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTgccgc                                   >  1:460316/1‑81 (MQ=255)                                     gCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTgccg                                    <  1:460278/80‑1 (MQ=255)                                       ccGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGccc                                 <  2:460279/81‑1 (MQ=255)                                       ccGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGccc                                 <  2:460281/81‑1 (MQ=255)                                            ttACGCGCCACGCAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCCACAcc                            <  1:460282/81‑1 (MQ=255)                                            ttACGCGCCACGCAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCCACAcc                            <  2:460283/81‑1 (MQ=255)                                                  gcCACGCAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCCACACCAAAGAc                      <  1:460285/81‑1 (MQ=255)                                                  gcCACGCAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCCACACCAAAGAc                      <  2:460284/81‑1 (MQ=255)                                                  gcCACGCAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCCACACCAAAGAc                      >  1:460330/1‑81 (MQ=255)                                                      cGCAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCCACACCAAAGACGTCg                  <  2:460286/81‑1 (MQ=255)                                                        cAGAAATTTTGGTTATTACAGATGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCCACACCAAAGACGTCGGc                <  1:460287/81‑1 (MQ=255)                                                           aaaTTTTGGTTATTACAGATGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCCACACCAAAGACGTCGGcaac             <  2:460288/81‑1 (MQ=255)                                                             aTTTTGGTTATTACAGATGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCCACACCAAAGACGTCGGcaacaa           <  2:460289/81‑1 (MQ=255)                                                                ttGGTTATTACAGATGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCCACACCAAAGACGTCGGCAACAAAg         <  1:460290/80‑1 (MQ=255)                                                                    ttattaCAGATGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCCACACCAAAGACGTCGGCAACAAAGCGaaa    <  2:460291/81‑1 (MQ=255)                                                                      attaCAGATGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCCACACCAAAGACGTCGGAAACAAAGCGaaaaa  <  2:460292/81‑1 (MQ=255)                                                                             |                                                                          GCTGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCCACACCAAAGACGTCGGCAACAAAGCGAAAAA  >  NC_000913/2582601‑2582750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑