Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,595,512	A→G	P116P (CCT→CCC)	tmcA ←	tRNA(Met) cytidine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,595,512	0	A	G	100.0%	80.8 / NA	24	P116P (CCT→CCC)	tmcA	tRNA(Met) cytidine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/6);  total (18/6)

CGTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCATCAGGTTGGTTTTCCCACTCTTCCCATACAGGGAGTAACAAAACCAGCCAGCTTCCCGCTTTCAACGTTCCGCTAAGTGC  >  NC_000913/2595438‑2595589                                                                           |                                                                              cgTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCATCGggttgg                                                                         >  2:463017/1‑81 (MQ=255)  gTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCATCGggttggt                                                                        >  2:731452/1‑81 (MQ=255)  gTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCATCGggttggt                                                                        >  1:463016/1‑81 (MQ=255)      gagaTGCTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCATCGGGTTGGtttt                                                                     <  1:462985/80‑1 (MQ=255)      gagaTGCTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCATCGGGTTGGTTTTc                                                                    >  2:463032/1‑81 (MQ=255)      gagaTGCTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCATCGGGTTGGTTTTc                                                                    >  1:463011/1‑81 (MQ=255)       agaTGCTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCATCGGGTTGGTTTTcc                                                                   >  2:463018/1‑81 (MQ=255)                aCAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCATCGGGTTGGTTTTCCCACTCTTcc                                                          >  2:463026/1‑81 (MQ=255)                aCAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCATCGGGTTGGTTTTCCCACTCTTcc                                                          >  1:463005/1‑81 (MQ=255)                  aaaaTGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCATCGGGTTGGTTTTCCCACTCTTCCCa                                                        >  1:463021/1‑81 (MQ=255)                   aaaTGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGACATCGGGTTGGTTTTCCCACTCTTCCCAt                                                       <  1:462986/81‑1 (MQ=255)                             tCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCATCGGGTTGGTTTTCCCACTCTTCCCATACAGGGAGTa                                             <  1:462987/81‑1 (MQ=255)                              cGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCATCGGGTTGGTTTTCCCACTCTTCCCATACAGGGAGTaa                                            >  1:463027/1‑81 (MQ=255)                                cAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCATCGGGTTGGTTTTCCCACTCTTCCCATACAGGGAGTAAc                                           >  1:463023/1‑80 (MQ=255)                                 aaTAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCATCGGGTTGGTTTTCCCACTCTTCCCATACAGGGAGTAACa                                          >  2:463024/1‑80 (MQ=255)                                     gggTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCATCGGGTTGGTTTTCCCACTCTTCCCATACAGGGAGTAACAAAAc                                      >  1:463030/1‑80 (MQ=255)                                                    cTCCAGCGCAGCGAGTCGGCATCGGGTTGGTTTTCCCACTCTTCCCATACAGGGAGTAACAAAACCAGCCAGCTTCCCGCt                      >  2:463034/1‑81 (MQ=255)                                                         gcgcagcgAGTCGGCATCGGGTTGGTTTTCCCACTCTTCCCATACAGGGAGTAACAAAACCAGCCAGCTTCCCGCTTTCaa                 <  1:462988/81‑1 (MQ=255)                                                           gcagcgAGTCGGCATCGGGTTGGTTTTCCCACTCTTCCCATACAGGGAGTAACAAAACCAGCCAGCTTCCCGCTTTCAACg               <  1:462989/81‑1 (MQ=255)                                                             agcgAGTCGGCATCGGGTTGGTTTTCCCACTCTTCCCATACAGGGAGTAACAAAACCAGCCAGCTTCCCGCTTTCAACGtt             >  2:463029/1‑81 (MQ=255)                                                                   tCGGCATCGGGTTGGTTTTCCCACTCTTCCCATACAGGGAGTAACAAAACCAGCCAGCTTCCCGCTTTCAACGTTCCGCt        >  2:463031/1‑80 (MQ=255)                                                                     ggCATCGGGTTGGTTTTCCCACTCTTCCCATACAGGGAGTAACAAAACCAGCCAGCTTCCCGCTTTCAACGTTCCGCTAAg     >  1:463035/1‑81 (MQ=255)                                                                      gCATCGGGTTGGTTTTCCCACTCTTCCCATACAGGGAGTAACAAAACCAGCCAGCTTCCCGCTTTCAACGTTCCGCTAAGt    <  1:462990/81‑1 (MQ=255)                                                                        aTCGGGTTGGTTTTCCCACTCTTCCCATACAGGGAGTAACAAAACCAGCCAGCTTCCCGCTTTCAACGTTCCGCTAAGTGc  >  2:463053/1‑81 (MQ=255)                                                                           |                                                                              CGTTTGAGATGCTGGACAAAATGCGGCGTCGCAATAGGGTCAGGGCAATCACTCCAGCGCAGCGAGTCGGCATCAGGTTGGTTTTCCCACTCTTCCCATACAGGGAGTAACAAAACCAGCCAGCTTCCCGCTTTCAACGTTCCGCTAAGTGC  >  NC_000913/2595438‑2595589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑