Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	293,374	A→G	V182A (GTT→GCT)	yagL ←	CP4‑6 prophage; resolvase‑like catalytic domain‑containing protein YagL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	293,374	0	A	G	100.0%	73.4 / NA	22	V182A (GTT→GCT)	yagL	CP4‑6 prophage; resolvase‑like catalytic domain‑containing protein YagL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/10);  total (12/10)

GAGTAAATCCACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATCAAAAAA  >  NC_000913/293297‑293445                                                                              |                                                                        gAGTAAATCCACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCAt                                                                      >  2:68282/1‑81 (MQ=255)     aaaTCCACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTc                                                                   >  1:68277/1‑80 (MQ=255)          cACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATc                                                             >  2:68286/1‑81 (MQ=255)          cACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATc                                                             >  2:68272/1‑81 (MQ=255)           aCCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCg                                                            >  2:68284/1‑81 (MQ=255)               ttCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCtt                                                        >  2:68281/1‑81 (MQ=255)                     aGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGtt                                                  >  2:68280/1‑81 (MQ=255)                         ttGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGAtt                                              <  2:68244/81‑1 (MQ=255)                         ttGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGAt                                               <  2:68243/80‑1 (MQ=255)                           gtgATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGAttt                                             <  1:68245/80‑1 (MQ=255)                            tgATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTc                                            <  2:68246/80‑1 (MQ=255)                             gATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGAttt                                             >  1:68279/1‑78 (MQ=255)                                   aCGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATtgt                                     <  1:68247/80‑1 (MQ=255)                                        ggTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGt                               >  2:68291/1‑81 (MQ=255)                                        ggTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGt                               >  2:68278/1‑81 (MQ=255)                                            tttGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAAt                           <  1:68248/81‑1 (MQ=255)                                              tGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATc                          <  1:68249/80‑1 (MQ=255)                                               gCTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCg                         <  2:68250/80‑1 (MQ=255)                                                cTTCCCTCCTTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGt                        <  2:68251/80‑1 (MQ=255)                                                      tcctTTCGTCCTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGa                 >  2:68290/1‑81 (MQ=255)                                                               ccTAATTTTTTCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAAt         >  2:68293/1‑80 (MQ=255)                                                                      ttttCTTAGCATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATCaaaaaa  <  2:68252/80‑1 (MQ=255)                                                                              |                                                                        GAGTAAATCCACCTTTCTTAAGTATTGTGATATCACGTCGGTATTTGCTTCCCTCCTTTCGTCCTAATTTTTTCTTAACATGTCGATATCGGCTTTTAGTTGATTTCAGATTGTGAATGTCAATCGTATCCTGACGAGCAATCAAAAAA  >  NC_000913/293297‑293445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑