Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,601,519	G→A	A107T (GCC→ACC)	hyfA →	hydrogenase 4 component A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,601,519	0	G	A	100.0%	130.4 / NA	36	A107T (GCC→ACC)	hyfA	hydrogenase 4 component A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/15);  total (21/15)

CTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTG  >  NC_000913/2601441‑2601596                                                                               |                                                                              ctctGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATAcc                                                                             >  1:464217/1‑81 (MQ=255)   ctGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCa                                                                           >  1:464223/1‑81 (MQ=255)      tATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCAt                                                                          <  1:464170/79‑1 (MQ=255)      tATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCAt                                                                          <  1:464169/79‑1 (MQ=255)         tGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATgcgc                                                                      >  1:464212/1‑80 (MQ=255)               cAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGt                                                               >  2:464224/1‑81 (MQ=255)                aaGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGtt                                                              <  2:464171/81‑1 (MQ=255)                    tttGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGttttt                                                           >  2:464225/1‑80 (MQ=255)                      tGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAg                                                        >  2:464220/1‑81 (MQ=255)                        cgcCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGc                                                      <  1:464172/81‑1 (MQ=255)                         gcCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCt                                                     <  1:464174/81‑1 (MQ=255)                         gcCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCt                                                     <  1:464173/81‑1 (MQ=255)                          ccGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTg                                                    <  2:464175/81‑1 (MQ=255)                             tGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAg                                                 <  1:464176/81‑1 (MQ=255)                                tCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGct                                              >  2:464227/1‑81 (MQ=255)                                    cccATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGctc                                             <  1:464177/78‑1 (MQ=255)                                      cATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGctcactc                                         >  2:464221/1‑80 (MQ=255)                                        tttGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGctcactca                                        >  1:464215/1‑79 (MQ=255)                                            gcgcAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACg                                  <  2:464178/81‑1 (MQ=255)                                               cAATCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGc                                >  1:464235/1‑80 (MQ=255)                                                 aTCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCgaa                             <  2:464179/81‑1 (MQ=255)                                                  tCAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCgaag                            <  1:464180/81‑1 (MQ=255)                                                   cAGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCgaag                            >  2:464222/1‑80 (MQ=255)                                                    aGCGCTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCgaaga                           >  2:464232/1‑80 (MQ=255)                                                      cgcTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGaaaa                        >  1:464207/1‑81 (MQ=255)                                                      cgcTTCAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGaaaa                        >  2:464243/1‑81 (MQ=255)                                                           cAGGAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACgcgc                   >  2:464259/1‑81 (MQ=255)                                                             ggAAGCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCca                 <  2:464181/81‑1 (MQ=255)                                                                 gCCGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCcaaca              >  1:464242/1‑80 (MQ=255)                                                                  ccGTCCGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCcaacaca            <  1:464182/81‑1 (MQ=255)                                                                       cGGTGAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATg       >  1:464229/1‑81 (MQ=255)                                                                           gAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCttt   >  1:464228/1‑81 (MQ=255)                                                                           gAATACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCttt   >  2:464257/1‑81 (MQ=255)                                                                            aaTACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCttt   <  1:464183/80‑1 (MQ=255)                                                                            aaTACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTtg  >  1:464244/1‑81 (MQ=255)                                                                            aaTACCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTtg  >  2:464237/1‑81 (MQ=255)                                                                               |                                                                              CTCTGTATTGGCTGCAAGCTTTGCGCCGTGGTCTGCCCATTTGGCGCAATCAGCGCTTCAGGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTG  >  NC_000913/2601441‑2601596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑