Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,607,268	T→C	F118L (TTC→CTC)	hyfF →	hydrogenase 4 component F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,607,268	0	T	C	100.0%	105.0 / NA	32	F118L (TTC→CTC)	hyfF	hydrogenase 4 component F
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/12);  total (20/12)

TACTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGGCGGCGATCGA  >  NC_000913/2607193‑2607344                                                                            |                                                                             tACTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTctctt                                                                          <  1:465449/80‑1 (MQ=255)   cTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTcc                                                                        <  2:465450/80‑1 (MQ=255)        ttGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGt                                                                  >  1:465503/1‑81 (MQ=255)           gCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGttt                                                                <  1:465451/80‑1 (MQ=255)             tACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTg                                                             >  2:465485/1‑81 (MQ=255)                     tCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCAt                                                     <  1:465452/81‑1 (MQ=255)                        tGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCAtgct                                                  <  2:465453/81‑1 (MQ=255)                         gAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCAtgctg                                                 >  2:465489/1‑81 (MQ=255)                         gAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCAtgctg                                                 >  1:465482/1‑81 (MQ=255)                          aaGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCAATCTGTTTTTGTTCACCAtgctgc                                                >  1:465507/1‑81 (MQ=255)                            gTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGctgctg                                              >  2:465495/1‑81 (MQ=255)                                cacaCGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGgttg                                          <  1:465454/81‑1 (MQ=255)                                 acacGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGgttgt                                         <  2:465455/81‑1 (MQ=255)                                    cGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTAc                                      <  2:465456/81‑1 (MQ=255)                                     ggCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTAc                                      >  1:465506/1‑80 (MQ=255)                                      gCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCCTCCGTCTTTGTTCACCATGCTGCTGGTTGTTAc                                      >  1:465505/1‑79 (MQ=255)                                        gAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAg                                   >  2:465487/1‑80 (MQ=255)                                        gAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGc                                  <  2:465457/81‑1 (MQ=255)                                              tCACCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAAc                            >  2:465500/1‑81 (MQ=255)                                                aCCCGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCt                          >  1:465510/1‑81 (MQ=255)                                                 cccGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCt                          >  1:465512/1‑80 (MQ=255)                                                  ccGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGa                        >  2:465494/1‑81 (MQ=255)                                                   cGTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGAt                       >  1:465499/1‑81 (MQ=255)                                                    gTTACGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGAtt                      >  2:465492/1‑81 (MQ=255)                                                       aCGCTGTGCGATTACTACTGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATtgtg                   >  2:465502/1‑81 (MQ=255)                                                       aCGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATtgt                    <  2:465458/80‑1 (MQ=255)                                                       aCGCTGTGCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATtgt                    >  2:465493/1‑80 (MQ=255)                                                              gCGATTACTACGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTggg            >  2:465459/1‑81 (MQ=255)                                                                    actacGGTCTCTTCCAACTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGgcggcg       <  1:465460/80‑1 (MQ=255)                                                                        cGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGGCGGCGATc    <  1:465462/79‑1 (MQ=255)                                                                        cGGTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGGCGGCGATCGa  <  1:465461/81‑1 (MQ=255)                                                                         ggTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGGCGGCGATCg   >  2:465513/1‑79 (MQ=255)                                                                         ggTCTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGGCGGCGATCg   >  2:465524/1‑79 (MQ=255)                                                                            |                                                                             TACTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGGCGGCGATCGA  >  NC_000913/2607193‑2607344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑