Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,629,977	C→T	N62N (AAC→AAT)	yfgH →	lipoprotein YfgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,629,977	0	C	T	100.0%	108.9 / NA	31	N62N (AAC→AAT)	yfgH	lipoprotein YfgH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/13);  total (18/13)

CGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGT  >  NC_000913/2629899‑2630054                                                                               |                                                                              cGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAATTc                                                                             <  1:469775/81‑1 (MQ=255) cGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATAATATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAATTc                                                                             <  2:469776/81‑1 (MQ=255)   aTCAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAATTcc                                                                            >  1:469815/1‑80 (MQ=255)     cAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAATTCCCa                                                                          >  1:469796/1‑80 (MQ=255)      aaCTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAATTCCCaa                                                                         >  1:469823/1‑80 (MQ=255)            aTACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAATTCCCAAAATAAAc                                                                  <  2:469777/81‑1 (MQ=255)                 aaaCAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAATTCCCAAAATAAACGGAAc                                                             <  1:469778/81‑1 (MQ=255)                  aaCAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAATTCCCAAAATAAACGGAACg                                                            >  1:469812/1‑81 (MQ=255)                   aCAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAATTCCCAAAATAAACGGAACGc                                                           <  2:469779/81‑1 (MQ=255)                        aaaCTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAATTCCCAAAATAAACGGAACGCACAAg                                                      <  2:469780/81‑1 (MQ=255)                             aaaaCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAATTCCCAAAATAAACGGAACGCACAAGCCTTc                                                 >  2:469811/1‑81 (MQ=255)                                aCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAATTCCCAAAATAAACGGAACGCACAAGCCTTCGgc                                              >  2:469808/1‑81 (MQ=255)                                aCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAATTCCCAAAATAAACGGAACGCACAAGCCTTCGgc                                              >  1:469805/1‑81 (MQ=255)                                aCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAATTCCCAAAATAAACGGAACGCACAAGCCTTCGgc                                              >  2:469818/1‑81 (MQ=255)                                aCCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAATTCCCAAAATAAACGGAACGCACAAGCCTTCGgc                                              <  2:469781/81‑1 (MQ=255)                                  cGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAATTCCCAAAATAAACGGAACGCACAAGCCTTCGgcgc                                            <  1:469782/81‑1 (MQ=255)                                   gTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAATTCCCAAAATAAACGGAACGCACAAGCCTTCGgcgcg                                           >  1:469801/1‑81 (MQ=255)                                    ttAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAATTCCCAAAATAAACGGAACGCACAAGCCTTCGgcgcgc                                          >  1:469819/1‑81 (MQ=255)                                       atatTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAATTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCtt                                        <  1:469783/80‑1 (MQ=255)                                          ttattTCCATTCTTCCCGCAAAAGTTGCCGTAGACAATTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTAtt                                     <  2:469784/80‑1 (MQ=255)                                            attTCCATTCTTCCCGCAAAAGTTGCCGTAGACAATTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGgc                                  >  1:469813/1‑81 (MQ=255)                                                     cttcCCGCAAAAGTTGCCGTAGACAATTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCt                         >  1:469836/1‑81 (MQ=255)                                                        cccGCAAAAGTTGCCGTAGACAATTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGc                      >  1:469814/1‑81 (MQ=255)                                                         ccGCAAAAGTTGCCGTAGACAATTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCg                     >  1:469817/1‑81 (MQ=255)                                                             aaaaGTTGCCGTAGACAATTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGgtgt                 >  1:469827/1‑81 (MQ=255)                                                              aaaGTTGCCGTAGACAATTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGtt                <  2:469785/81‑1 (MQ=255)                                                                       gTAGACAATTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCcac       <  2:469786/81‑1 (MQ=255)                                                                       gTAGACAATTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCcac       <  2:469787/81‑1 (MQ=255)                                                                            cAATTCCCAAAATAAACGGAACGCACAAGCCTTCGGCTCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGt  >  1:469825/1‑81 (MQ=255)                                                                            cAATTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGt  >  1:469816/1‑81 (MQ=255)                                                                            cAATTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGt  >  2:469830/1‑81 (MQ=255)                                                                               |                                                                              CGATCAACTGAATACCAAACAAGAAACTAAAACCGTTAATATTATTTCCATTCTTCCCGCAAAAGTTGCCGTAGACAACTCCCAAAATAAACGGAACGCACAAGCCTTCGGCGCGCTTATTGGCGCAGTCGCTGGCGGTGTTATCGGCCACAACGT  >  NC_000913/2629899‑2630054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑