Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,632,630	A→G	S481P (TCC→CCC)	guaB ←	inosine 5'‑monophosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,632,630	0	A	G	100.0%	137.0 / NA	39	S481P (TCC→CCC)	guaB	inosine 5'‑monophosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/13);  total (26/13)

TGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTT  >  NC_000913/2632557‑2632704                                                                          |                                                                           tGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTa                                                                     <  1:470321/81‑1 (MQ=255)   aaaCAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGt                                                                   <  2:470322/81‑1 (MQ=255)     aCAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTaa                                                                 >  2:470362/1‑81 (MQ=255)        gATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATgg                                                              <  2:470323/81‑1 (MQ=255)          taataTAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTc                                                            <  1:470324/81‑1 (MQ=255)              ataAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCAcgt                                                        >  2:470364/1‑81 (MQ=255)               taAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCAcgtc                                                       <  1:470325/81‑1 (MQ=255)                 aaTCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCAcgtcgt                                                     >  1:470346/1‑81 (MQ=255)                   tCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTg                                                    >  1:470344/1‑80 (MQ=255)                   tCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGa                                                   <  2:470326/81‑1 (MQ=255)                    cGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGaa                                                  >  1:470357/1‑81 (MQ=255)                    cGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGaa                                                  >  1:470359/1‑81 (MQ=255)                    cGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGaa                                                  >  1:470360/1‑81 (MQ=255)                      cccGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACg                                                >  2:470352/1‑81 (MQ=255)                      cccGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACg                                                >  2:470347/1‑81 (MQ=255)                          aCATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGc                                            >  1:470356/1‑81 (MQ=255)                           cATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCt                                           <  2:470327/81‑1 (MQ=255)                            aTGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCtt                                          <  1:470328/81‑1 (MQ=255)                              gAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTc                                        >  1:470374/1‑81 (MQ=255)                                   cGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGaa                                   >  2:470373/1‑81 (MQ=255)                                   cGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGaa                                   >  1:470349/1‑81 (MQ=255)                                    gggCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAAt                                  >  2:470350/1‑81 (MQ=255)                                      gCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGc                                >  1:470361/1‑81 (MQ=255)                                       cGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGcc                               >  2:470367/1‑81 (MQ=255)                                         aaGAGAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCg                             >  1:470382/1‑81 (MQ=255)                                          agagAATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCg                             >  1:470378/1‑80 (MQ=255)                                            agaATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCAc                          <  1:470329/81‑1 (MQ=255)                                             gaATCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCAcc                         <  2:470330/81‑1 (MQ=255)                                               aTCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGc                       >  1:470355/1‑81 (MQ=255)                                               aTCAGGAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGc                       >  2:470379/1‑81 (MQ=255)                                                   ggAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCtta                    >  2:470365/1‑78 (MQ=255)                                                   ggAGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGa                    >  1:470363/1‑80 (MQ=255)                                                     aGCCCAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATAc                 <  1:470331/81‑1 (MQ=255)                                                       cccAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGt               <  2:470333/81‑1 (MQ=255)                                                       cccAGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGt               <  2:470332/81‑1 (MQ=255)                                                          aGACGGTAGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTAc             >  1:470369/1‑80 (MQ=255)                                                                 aGTTCGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCg     >  2:470385/1‑81 (MQ=255)                                                                     cGGGGGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCtt  >  2:470372/1‑80 (MQ=255)                                                                      ggggGCTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCtt  >  1:470380/1‑79 (MQ=255)                                                                          |                                                                           TGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTT  >  NC_000913/2632557‑2632704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑