Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,637,925	C→A	R243L (CGT→CTT)	bamB ←	outer membrane protein assembly factor BamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,637,925	0	C	A	100.0%	135.7 / NA	38	R243L (CGT→CTT)	bamB	outer membrane protein assembly factor BamB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/20);  total (18/20)

GTCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGC  >  NC_000913/2637850‑2638001                                                                            |                                                                             gTCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTc                                                                          >  2:471530/1‑80 (MQ=255)  tCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCaa                                                                        >  2:471528/1‑81 (MQ=255)  tCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCaa                                                                        <  2:471465/81‑1 (MQ=255)    aGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAAtt                                                                      <  2:471466/81‑1 (MQ=255)    aGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAAt                                                                       <  1:471467/80‑1 (MQ=255)     ggTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAAttt                                                                     >  2:471520/1‑81 (MQ=255)         aCCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCgg                                                                  <  1:471468/80‑1 (MQ=255)           cATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAg                                                               <  2:471469/81‑1 (MQ=255)                taGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCg                                                          >  1:471514/1‑81 (MQ=255)                  ggCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCgg                                                         <  1:471470/80‑1 (MQ=255)                   gCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTc                                                       <  2:471471/81‑1 (MQ=255)                      aGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGcc                                                    >  1:471523/1‑81 (MQ=255)                       gcgcGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCt                                                   <  2:471472/81‑1 (MQ=255)                        cgcgAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTg                                                  >  1:471512/1‑81 (MQ=255)                         gcgAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTgg                                                 >  2:471513/1‑81 (MQ=255)                          cgAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTggg                                                <  1:471473/81‑1 (MQ=255)                          cgAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTggg                                                <  1:471474/81‑1 (MQ=255)                           gAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTggg                                                >  2:471532/1‑80 (MQ=255)                           gAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTggg                                                >  2:471534/1‑80 (MQ=255)                            aaaaCAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGaa                                              <  1:471475/81‑1 (MQ=255)                                          aacaacGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGctgctgtc                                 >  1:471527/1‑78 (MQ=255)                                           acaacGACGGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCa                                <  1:471476/80‑1 (MQ=255)                                                gacgGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAatcat                          >  2:471546/1‑81 (MQ=255)                                                gacgGGAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAatcat                          <  1:471477/81‑1 (MQ=255)                                                   gggAGTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTg                       <  2:471478/81‑1 (MQ=255)                                                       gTCGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGccc                   >  2:471533/1‑81 (MQ=255)                                                         cGTGTCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTg                 >  2:471526/1‑81 (MQ=255)                                                          gtgtCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGTAATACGCTGCTGCCAAATCATCTGGCCCTGt                >  2:471524/1‑81 (MQ=255)                                                            gtCAACATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTc              >  1:471525/1‑81 (MQ=255)                                                               aaCATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCAt           >  1:471535/1‑81 (MQ=255)                                                               aaCATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCAt           >  2:471544/1‑81 (MQ=255)                                                                aCATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATc          <  2:471479/81‑1 (MQ=255)                                                                 cATCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCa         <  1:471480/81‑1 (MQ=255)                                                                   tCGCTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGc       <  1:471481/81‑1 (MQ=255)                                                                      cTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACt    <  2:471483/81‑1 (MQ=255)                                                                      cTCAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACt    <  1:471482/81‑1 (MQ=255)                                                                        cAGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTgc  >  1:471551/1‑81 (MQ=255)                                                                         aGAAGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTg   <  1:471484/79‑1 (MQ=255)                                                                            |                                                                             GTCAGGTTACCATTATAGGCCAGCGCGAAAACAACGCCGTTAACAACGACGGGAGTCGTGTCAACATCGCTCAGACGGTCAATTTCGGTAGAACCGGTCGCCTGGGAAATACGCTGCTGCCAAATCATCTGGCCCTGTTCCATCAGCACTGC  >  NC_000913/2637850‑2638001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑