Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	298,160	C→G	A189A (GCG→GCC)	paoD ←	molybdenum cofactor insertion chaperone for PaoABC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	298,160	0	C	G	100.0%	134.8 / NA	39	A189A (GCG→GCC)	paoD	molybdenum cofactor insertion chaperone for PaoABC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (22/17);  total (22/17)

AGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTC  >  NC_000913/298082‑298232                                                                               |                                                                         aGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTgggc                                                                        <  1:68996/81‑1 (MQ=255)    gctggctgAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCt                                                                      >  2:69055/1‑80 (MQ=255)    gctggctgAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTc                                                                     <  1:68997/81‑1 (MQ=255)    gctggctgAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTc                                                                     <  2:68998/81‑1 (MQ=255)     ctggctgAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCa                                                                    <  2:68999/81‑1 (MQ=255)          tgAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAagag                                                                >  2:69075/1‑80 (MQ=255)           gAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGaa                                                              >  2:69042/1‑81 (MQ=255)            aGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGaa                                                              <  2:69000/80‑1 (MQ=255)            aGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAAc                                                             >  2:69060/1‑81 (MQ=255)             ggCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACg                                                            >  2:69057/1‑81 (MQ=255)               ccGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTc                                                          <  1:69001/81‑1 (MQ=255)                cGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTcc                                                         <  1:69002/81‑1 (MQ=255)                  gAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGt                                                       <  1:69003/81‑1 (MQ=255)                   aaaaaGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTa                                                      <  1:69004/81‑1 (MQ=255)                    aaaaGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTa                                                      >  1:69058/1‑80 (MQ=255)                          tCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAatcatc                                               <  2:69005/81‑1 (MQ=255)                               gATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCt                                          >  2:69071/1‑81 (MQ=255)                                  atGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGAc                                       >  1:69088/1‑81 (MQ=255)                                   tGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGAc                                       <  2:69007/80‑1 (MQ=255)                                   tGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGAc                                       <  1:69006/80‑1 (MQ=255)                                     gCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGc                                     <  2:69008/80‑1 (MQ=255)                                     gCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCa                                    >  1:69047/1‑81 (MQ=255)                                     gCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCa                                    >  2:69064/1‑81 (MQ=255)                                       tGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATg                                  >  1:69062/1‑81 (MQ=255)                                         tCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATgg                                 <  2:69009/80‑1 (MQ=255)                                         tCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGc                                >  1:69056/1‑81 (MQ=255)                                             aGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTg                             >  2:69069/1‑80 (MQ=255)                                              gCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGaa                           >  1:69076/1‑81 (MQ=255)                                              gCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGa                            <  1:69010/80‑1 (MQ=255)                                                cTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAAcc                         >  1:69067/1‑81 (MQ=255)                                                cTGTGGCTGCTGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAAcc                         >  1:69030/1‑81 (MQ=255)                                                     gctgctGCAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCAcc                    >  1:69036/1‑81 (MQ=255)                                                          tgcAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCa                 >  2:69066/1‑79 (MQ=255)                                                            cAAGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAg              <  2:69011/80‑1 (MQ=255)                                                             aaGACTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGc             >  2:69063/1‑80 (MQ=255)                                                                aCTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCAtt         <  1:69013/81‑1 (MQ=255)                                                                aCTCGCGGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCAtt         <  1:69012/81‑1 (MQ=255)                                                                   cgcgGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATAAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCAtt         >  1:69046/1‑78 (MQ=255)                                                                    gcgGTTGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCAtt         >  1:69050/1‑77 (MQ=255)                                                                         tGCCTGGGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTc  >  2:69065/1‑79 (MQ=255)                                                                               |                                                                         AGCGCTGGCTGAGGCCGGAAAAAGATCGAAGATATGGCTGTCATAGCCTGTGGCTGCTGCAAGACTCGCGGTTGCCTGCGCCTCAAGAGAACGTCCGTAAATCATCAGCCTGACGCATGGCCTGAACCCCACCTCAAAGCCATTGAGATTC  >  NC_000913/298082‑298232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑