Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,645,776	C→T	G517D (GGC→GAC)	pbpC ←	peptidoglycan glycosyltransferase PbpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,645,776	0	C	T	100.0%	109.0 / NA	31	G517D (GGC→GAC)	pbpC	peptidoglycan glycosyltransferase PbpC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/11);  total (20/11)

GCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCA  >  NC_000913/2645699‑2645853                                                                              |                                                                              gCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGTCAt                                                                            <  2:473196/81‑1 (MQ=255)        gTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGTCATAGCTGGt                                                                     >  2:473274/1‑81 (MQ=255)         tCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGTCATAGCTGGTg                                                                    <  2:473197/81‑1 (MQ=255)          ccGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGTCATAGCTGGTGc                                                                   >  1:473250/1‑81 (MQ=255)           cGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGTCATAGCTGGTGcc                                                                  >  2:473235/1‑81 (MQ=255)            ggTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGTCATAGCTGGTGccc                                                                 >  2:473240/1‑81 (MQ=255)                    aGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGTCATAGCTGGTGCCCGTTTTCCa                                                         >  1:473246/1‑81 (MQ=255)                        cAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGTCATAGCTGGTGCCCGTTTTCCATGcc                                                     <  1:473198/81‑1 (MQ=255)                        cAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGTCATAGCTGGTGCCCGTTTTCCATGc                                                      <  2:473199/80‑1 (MQ=255)                                 aTGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGTCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCg                                            <  2:473200/81‑1 (MQ=255)                                      aTAGCGAGCGTTAACCCCAATCGCCCATGCATCACGATAGTCTTAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGAcgcg                                       >  1:473265/1‑81 (MQ=255)                                      aTAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGTCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGAcgcg                                       >  1:473247/1‑81 (MQ=255)                                        agcgagcgTTAACCCCAATCGCCCAGGCGTCACGATAGTCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGAcgcgcg                                     >  1:473257/1‑81 (MQ=255)                                        agcgagcgTTAACCCCAATCGCCCAGGCGTCACGATAGTCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGAcgcgcg                                     >  1:473262/1‑81 (MQ=255)                                          cgagcgTTAACCCCAATCGCCCAGGCGTCACGATAGTCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGc                                   <  2:473201/81‑1 (MQ=255)                                                  aaCCCCAATCGCCCAGGCGTCACGATAGTCATAGCTGGTGCCCGTTTTCCATGCCAGTGGTGCGACGCGCGGCAAGGCAc                            >  1:473252/1‑80 (MQ=255)                                                  aaCCCCAATCGCCCAGGCGTCACGATAGTCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCAc                            <  2:473202/80‑1 (MQ=255)                                                  aaCCCCAATCGCCCAGGCGTCACGATAGTCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCAc                            >  2:473269/1‑80 (MQ=255)                                                   aCCCCAATCGCCCAGGCGTCACGATAGTCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACt                           <  1:473203/80‑1 (MQ=255)                                                        aaTCGCCCAGGCGTCACGATAGTCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCgg                     >  2:473254/1‑81 (MQ=255)                                                         aTCGCCCAGGCGTCACGATAGTCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCgg                     >  2:473256/1‑80 (MQ=255)                                                         aTCGCCCAGGCGTCACGATAGTCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCgg                     >  1:473244/1‑80 (MQ=255)                                                               cAGGCGTCACGATAGTCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGt              <  2:473204/81‑1 (MQ=255)                                                                aGGCGTCACGATAGTCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGtt             >  2:473264/1‑81 (MQ=255)                                                                 ggCGTCACGATAGTCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTg            >  1:473251/1‑81 (MQ=255)                                                                  gCGTCACGATAGTCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTgc           <  1:473205/81‑1 (MQ=255)                                                                    gTCACGATAGTCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTgcgc         >  2:473280/1‑81 (MQ=255)                                                                     tCACGATAGTCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCt        >  1:473273/1‑81 (MQ=255)                                                                      cACGATAGTCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCtt       >  1:473258/1‑81 (MQ=255)                                                                       aCGATAGTCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCtt       >  2:473255/1‑80 (MQ=255)                                                                           tAGTCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTtcatca  <  2:473206/81‑1 (MQ=255)                                                                              |                                                                              GCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCA  >  NC_000913/2645699‑2645853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑