Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,693,373	G→C	A724G (GCC→GGC)	purL ←	phosphoribosylformylglycinamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,693,373	0	G	C	100.0%	125.3 / NA	37	A724G (GCC→GGC)	purL	phosphoribosylformylglycinamide synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (20/17);  total (20/17)

CACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCT  >  NC_000913/2693299‑2693448                                                                           |                                                                            cACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTgcgccg                                                                           <  2:483371/77‑1 (MQ=255) cACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGAt                                                                         <  1:483372/79‑1 (MQ=255) cACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATg                                                                        <  2:483370/80‑1 (MQ=255)  aCCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGtt                                                                      <  2:483373/81‑1 (MQ=255)    cAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTgg                                                                    <  1:483374/81‑1 (MQ=255)    cAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTgg                                                                    >  2:483426/1‑81 (MQ=255)     aGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGt                                                                   <  1:483375/81‑1 (MQ=255)         tGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAAc                                                               <  1:483376/81‑1 (MQ=255)           gCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACg                                                              <  1:483377/80‑1 (MQ=255)           gCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGc                                                             >  2:483441/1‑81 (MQ=255)                ccgccgccATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCac                                                        >  1:483422/1‑81 (MQ=255)                     gccATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCaccgacc                                                   >  1:483413/1‑81 (MQ=255)                        aTCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGcc                                                <  2:483378/81‑1 (MQ=255)                           cAGTTGGTGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGa                                             >  2:483430/1‑81 (MQ=255)                             gTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACg                                           <  2:483379/81‑1 (MQ=255)                             gTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACg                                           >  2:483434/1‑81 (MQ=255)                               tGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACggg                                         >  2:483420/1‑81 (MQ=255)                                ggCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACggg                                         >  1:483427/1‑80 (MQ=255)                                  cGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCag                                      <  2:483380/81‑1 (MQ=255)                                     aaaGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAgg                                   >  2:483429/1‑81 (MQ=255)                                       aGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAggcg                                 >  2:483439/1‑81 (MQ=255)                                            gATGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGa                             >  2:483418/1‑80 (MQ=255)                                             aTGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGaaa                           >  2:483433/1‑81 (MQ=255)                                              tGCGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAAt                          <  2:483381/81‑1 (MQ=255)                                                cGTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATc                         >  2:483425/1‑80 (MQ=255)                                                 gTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATc                         >  2:483396/1‑79 (MQ=255)                                                 gTTTGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATc                         >  1:483431/1‑79 (MQ=255)                                                  tttGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCcag                      >  2:483447/1‑81 (MQ=255)                                                   ttGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCcag                      >  1:483432/1‑80 (MQ=255)                                                    tGATATCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCcag                      >  2:483435/1‑79 (MQ=255)                                                       tatCGCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAgcg                 >  1:483452/1‑81 (MQ=255)                                                         tCGCCAATTTGTGTTGCGCCGATGTTGGTTAACACTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAgcgc                <  2:483382/80‑1 (MQ=255)                                                           gCCAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAAc             >  1:483446/1‑81 (MQ=255)                                                            ccAATTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAAcc            <  1:483383/81‑1 (MQ=255)                                                              aaTTTGTGTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCgg          <  2:483384/81‑1 (MQ=255)                                                                  tgtgTTGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGgcgc       <  1:483385/80‑1 (MQ=255)                                                                      ttGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAGATCCAGCAGCGCAACCAGCGCACACt  <  2:483386/81‑1 (MQ=255)                                                                      ttGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACt  <  2:483387/81‑1 (MQ=255)                                                                           |                                                                            CACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCT  >  NC_000913/2693299‑2693448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑