Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,693,446	G→A	R700C (CGT→TGT)	purL ←	phosphoribosylformylglycinamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,693,446	0	G	A	100.0%	157.7 / NA	44	R700C (CGT→TGT)	purL	phosphoribosylformylglycinamide synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/17);  total (27/17)

TTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCT  >  NC_000913/2693368‑2693523                                                                               |                                                                              ttGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAGATCCAGCAGCGCAACCAGCGCACACt                                                                             <  2:483386/81‑1 (MQ=255) ttGCGCCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACt                                                                             <  2:483387/81‑1 (MQ=255)    cgccgATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCg                                                                           <  1:483388/77‑1 (MQ=255)     gccgATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGc                                                                          >  2:483456/3‑80 (MQ=255)     gccgATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGc                                                                          >  1:483448/3‑80 (MQ=255)      ccgATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCa                                                                        <  1:483389/80‑1 (MQ=255)       cgATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCaa                                                                       <  1:483390/81‑1 (MQ=255)        gATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAAt                                                                      >  2:483458/1‑81 (MQ=255)        gATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAAt                                                                      >  1:483440/1‑81 (MQ=255)           gTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGc                                                                   >  2:483442/1‑81 (MQ=255)            ttGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGc                                                                   <  1:483391/80‑1 (MQ=255)            ttGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGc                                                                   >  2:483465/1‑80 (MQ=255)            ttGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGc                                                                   >  2:483436/1‑80 (MQ=255)              ggTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGCCAt                                                                >  2:483443/1‑81 (MQ=255)                ttAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGCCATCg                                                              <  1:483392/81‑1 (MQ=255)                         cACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGCCATCGCTTCACCg                                                      >  1:483462/1‑80 (MQ=255)                          accgaccgCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGCCATCGCTTCACCgt                                                     <  1:483394/80‑1 (MQ=255)                          accgaccgCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGCCATCGCTTCACCgt                                                     <  1:483393/80‑1 (MQ=255)                          accgaccgCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGCCATCGCTTCACCg                                                      >  1:483437/1‑79 (MQ=255)                           ccgaccgCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGTACACTCGCCAATCGCCATCGCTTCACCgtag                                                   >  2:483449/1‑81 (MQ=255)                             gaccgCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGCCATCGCTTCACCgtagta                                                 <  2:483395/81‑1 (MQ=255)                             gaccgCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGCCATCGCTTCACCgtag                                                   >  2:483453/1‑79 (MQ=255)                                cgCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCt                                              >  2:483444/1‑81 (MQ=255)                                 gCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCTg                                             >  2:483416/1‑81 (MQ=255)                                  ccAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGt                                            >  2:483450/1‑81 (MQ=255)                                   cAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTc                                           <  1:483396/81‑1 (MQ=255)                                    aGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCg                                          <  1:483397/81‑1 (MQ=255)                                         ggCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCt                                     >  1:483457/1‑81 (MQ=255)                                           cAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTgg                                   <  1:483398/81‑1 (MQ=255)                                              agGCGGCGAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCgg                                >  1:483480/1‑81 (MQ=255)                                                  ggcgAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGt                            >  2:483421/1‑81 (MQ=255)                                                    cgAAATCCAGCAGCGCAACCGGCGCACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGa                          >  2:483454/1‑81 (MQ=255)                                                          ccAGCAGCGCAACCGGCGCACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACcgcg                     >  1:483428/1‑80 (MQ=255)                                                              cagcGCAACCGGCGCACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGtt                <  1:483399/81‑1 (MQ=255)                                                               agcGCAACCGGCGCACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGt                 >  2:483468/1‑79 (MQ=255)                                                                 cgcAACCGGCGCACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGc             <  2:483400/81‑1 (MQ=255)                                                                  gcAACCGGCGCACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGc             >  2:483461/1‑80 (MQ=255)                                                                    aaCCGGCGCACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGAc          >  2:483464/1‑81 (MQ=255)                                                                    aaCCGGCGCACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGAc          <  1:483401/81‑1 (MQ=255)                                                                     aCCGGCGCACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGAcc         >  1:483467/1‑81 (MQ=255)                                                                     aCCGGCGCACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGAcc         >  1:483459/1‑81 (MQ=255)                                                                          cgcACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCAc    >  2:483469/1‑81 (MQ=255)                                                                           gcACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCAcc   >  1:483451/1‑81 (MQ=255)                                                                           gcACACTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCAcc   <  1:483402/81‑1 (MQ=255)                                                                            cacaCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCt  <  1:483404/81‑1 (MQ=255)                                                                            cacaCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCt  <  1:483403/81‑1 (MQ=255)                                                                               |                                                                              TTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCT  >  NC_000913/2693368‑2693523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑