Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,717,817	C→T	A238T (GCG→ACG)	yfiF ←	putative methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,717,817	0	C	T	100.0%	90.2 / NA	26	A238T (GCG→ACG)	yfiF	putative methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/12);  total (14/12)

TCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCAT  >  NC_000913/2717741‑2717889                                                                             |                                                                         tcCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGtcccc                                                                      <  1:488481/81‑1 (MQ=255) tcCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGtcccc                                                                      <  1:488480/81‑1 (MQ=255)  ccAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGtcccc                                                                      >  1:488482/1‑80 (MQ=255)     gCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACt                                                                  >  1:488534/1‑81 (MQ=255)          ttAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCcag                                                              >  1:488535/1‑80 (MQ=255)              cAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTg                                                         >  2:488533/1‑81 (MQ=255)                aTGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGcc                                                       <  2:488483/81‑1 (MQ=255)                       cGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCt                                                >  2:488538/1‑81 (MQ=255)                        gCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTg                                               <  2:488484/81‑1 (MQ=255)                              aaTCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGcaccac                                         >  2:488542/1‑81 (MQ=255)                                  ggCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGaca                                     >  2:488539/1‑81 (MQ=255)                                  ggCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGac                                      <  2:488485/80‑1 (MQ=255)                                   gCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGacac                                    <  1:488486/81‑1 (MQ=255)                                       aaCGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCttt                                <  1:488487/81‑1 (MQ=255)                                       aaCGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCt                                  <  1:488488/79‑1 (MQ=255)                                             ctctGCGCCGCCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGcc                          <  2:488489/81‑1 (MQ=255)                                                  cgccgcCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGa                        <  1:488491/78‑1 (MQ=255)                                                  cgccgcCTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGt                     <  1:488490/81‑1 (MQ=255)                                                     cgccTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTgcg                  <  1:488492/81‑1 (MQ=255)                                                       ccTTCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTgcgcg                >  1:488532/1‑81 (MQ=255)                                                         ttCTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTgcgcg                >  2:488550/1‑79 (MQ=255)                                                           cTGCGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGc            >  2:488544/1‑81 (MQ=255)                                                              cGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCt           <  1:488494/79‑1 (MQ=255)                                                              cGGTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTgc         <  1:488493/81‑1 (MQ=255)                                                               ggTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTgcg        >  2:488541/1‑81 (MQ=255)                                                                gTACGGATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTgcg        >  1:488537/1‑80 (MQ=255)                                                                    ggATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGcatca   >  1:488555/1‑81 (MQ=255)                                                                     gATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGcatcat  >  2:488553/1‑81 (MQ=255)                                                                     gATAGCCGTCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGcatcat  >  2:488558/1‑81 (MQ=255)                                                                             |                                                                         TCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCAT  >  NC_000913/2717741‑2717889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑