Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,731,100	G→T	noncoding (58/1542 nt)	rrsG ←	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,731,100	0	G	T	100.0%	56.8 / NA	17	noncoding (58/1542 nt)	rrsG	16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (4/13);  total (4/13)

GGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTT  >  NC_000913/2731022‑2731151                                                                               |                                                    ggCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTTCa                                                   >  2:491593/1‑81 (MQ=255)  gCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTTCAt                                                  >  1:491596/1‑81 (MQ=255)  gCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTTCAt                                                  <  2:491562/81‑1 (MQ=255)         cccAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTTCATGTGtt                                             <  2:491564/79‑1 (MQ=255)         cccAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTTCATGTGTTa                                            <  2:491563/80‑1 (MQ=255)          ccAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTTCATGTGTTAg                                           <  1:491565/80‑1 (MQ=255)           cAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTTCATGTGTTAGGc                                         <  1:491566/81‑1 (MQ=255)            aGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTTCATGTGTTAGGcc                                        <  1:491567/81‑1 (MQ=255)             gACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTTCATGTGTTAGGCCt                                       >  1:491592/1‑81 (MQ=255)               cATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTTCATGTGTTAGGCCTgc                                     >  1:491600/1‑81 (MQ=255)                    cTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTTCATGTGTTAGGCCTgccgcc                                 <  2:491568/80‑1 (MQ=255)                    cTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTTCATGTGTTAGGCCTgccgcc                                 <  2:491569/80‑1 (MQ=255)                    cTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTTCATGTGTTAGGCCTGCCGCCa                                <  1:491570/81‑1 (MQ=255)                                ccACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTTCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTg                    <  2:491571/81‑1 (MQ=255)                                      gTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTTCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGcc                <  1:491572/79‑1 (MQ=255)                                       tCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTTCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATg             <  1:491573/81‑1 (MQ=255)                                                  gcaAGCTGCTTCCTGTTACCGTTCGACTTTCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCtt  <  1:491574/81‑1 (MQ=18)                                                                               |                                                    GGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGACTTGCATGTGTTAGGCCTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTT  >  NC_000913/2731022‑2731151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑