Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,733,607	A→G	R189R (CGT→CGC)	clpB ←	ClpB chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,733,607	0	A	G	100.0%	113.7 / NA	33	R189R (CGT→CGC)	clpB	ClpB chaperone
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/21);  total (12/21)

GATGGCAGTTTTACCGACGCCGGGTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTACGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTCGGTAAGGTCGATGG  >  NC_000913/2733529‑2733680                                                                               |                                                                          gATGGCAGTTTTACCGACGCCGGGTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCg                                                                         <  1:492040/81‑1 (MQ=255)  aTGGCAGTTTTACCGACGCCGGGTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCgg                                                                        <  1:492041/81‑1 (MQ=255)    ggCAGTTTTACCGACGCCGGGTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGc                                                                       >  2:492087/1‑80 (MQ=255)         ttttACCGACGCCGGGTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAAttt                                                                 <  2:492042/81‑1 (MQ=255)           ttACCGACGCCGGGTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATttct                                                               >  1:492105/1‑81 (MQ=255)                gACGCCGGGTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTtcat                                                           >  2:492090/1‑80 (MQ=255)                  cGCCGGGTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCAc                                                        <  1:492043/81‑1 (MQ=255)                        gTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCaa                                                  <  1:492044/81‑1 (MQ=255)                         ttCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAAt                                                 >  2:492099/1‑81 (MQ=255)                          tCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAAt                                                 >  2:492108/1‑80 (MQ=255)                           cACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATc                                                <  1:492045/80‑1 (MQ=255)                           cACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATc                                                <  1:492046/80‑1 (MQ=255)                             ccAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCAcc                                             <  1:492047/81‑1 (MQ=255)                              cAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCg                                            >  2:492102/1‑81 (MQ=255)                              cAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCg                                            <  2:492048/81‑1 (MQ=255)                                aTCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGa                                          >  1:492110/1‑81 (MQ=255)                                   aGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATc                                        >  2:492096/1‑80 (MQ=255)                                     tACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAg                                     >  1:492107/1‑81 (MQ=255)                                              aTTTTTAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCt                             <  1:492049/80‑1 (MQ=255)                                               tttttAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGt                           <  2:492050/81‑1 (MQ=255)                                                 tttAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCAAGTTTGCCCTGtt                          <  2:492051/80‑1 (MQ=255)                                                   tAGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCg                        <  1:492052/80‑1 (MQ=255)                                                    aGTACGACGTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGc                      >  1:492103/1‑81 (MQ=255)                                                        cgacgTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGt                  <  1:492053/81‑1 (MQ=255)                                                         gacgTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGtt                 <  1:492055/81‑1 (MQ=255)                                                         gacgTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGtt                 <  2:492054/81‑1 (MQ=255)                                                         gacgTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGtt                 <  2:492056/81‑1 (MQ=255)                                                          acgTTGCAGCACCTGAATGGTGCGGCGAATTTCTGCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTc                <  1:492057/81‑1 (MQ=255)                                                           cgTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTCg               <  2:492058/81‑1 (MQ=255)                                                            gTTGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTCgg              >  2:492104/1‑81 (MQ=255)                                                             ttGCAGCACCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTCGGt             <  1:492059/81‑1 (MQ=255)                                                               gcagcaCCTGAATGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTCGGt             >  2:492101/1‑79 (MQ=255)                                                                         aaTGGTGCGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTCGGTAAGGTCGATgg  <  1:492060/80‑1 (MQ=255)                                                                               |                                                                          GATGGCAGTTTTACCGACGCCGGGTTCACCAATCAGTACCGGGTTATTTTTAGTACGACGTTGCAGCACCTGAATGGTACGGCGAATTTCTTCATCACGACCAATCACCGGATCGAGTTTGCCCTGTTCGGCTCGTTCGGTAAGGTCGATGG  >  NC_000913/2733529‑2733680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑