Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	304,754	C→T	R151Q (CGA→CAA)	ecpE ←	putative fimbrial chaperone EcpE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	304,754	0	C	T	100.0%	88.8 / NA	25	R151Q (CGA→CAA)	ecpE	putative fimbrial chaperone EcpE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/12);  total (13/12)

AACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTAT  >  NC_000913/304679‑304829                                                                            |                                                                            aaCCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGtact                                                                        <  1:70433/81‑1 (MQ=255) aaCCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGtac                                                                         <  1:70434/80‑1 (MQ=255)             ccAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGt                                                             <  2:70435/80‑1 (MQ=255)                   ttacttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCAt                                                       <  2:70436/80‑1 (MQ=255)                     acttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATcac                                                    >  2:70479/1‑81 (MQ=255)                     acttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATcac                                                    >  1:70478/1‑81 (MQ=255)                     acttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATcac                                                    >  1:70490/1‑81 (MQ=255)                      cttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATcacc                                                   <  1:70438/81‑1 (MQ=255)                      cttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATcacc                                                   <  2:70437/81‑1 (MQ=255)                           cGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATcaccaccac                                              >  2:70481/1‑81 (MQ=255)                                cTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGc                                          <  1:70439/80‑1 (MQ=255)                                cTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCt                                         >  1:70474/1‑81 (MQ=255)                                   tCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTcc                                       <  2:70440/80‑1 (MQ=255)                                          gTCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGAc                               >  1:70502/1‑81 (MQ=255)                                           tCGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGAcc                              <  1:70441/81‑1 (MQ=255)                                            cGAAGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCa                             >  2:70494/1‑81 (MQ=255)                                               aGGACCATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAcgc                          <  2:70443/81‑1 (MQ=255)                                                    cATTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGccgccg                     <  1:70444/81‑1 (MQ=255)                                                     aTTTAAACTGAACCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCgg                    >  1:70480/1‑81 (MQ=255)                                                               aaCCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCg          >  1:70489/1‑81 (MQ=255)                                                               aaCCTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCg          >  2:70508/1‑81 (MQ=255)                                                                  cTGACGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCt       >  1:70504/1‑81 (MQ=255)                                                                     aCGTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGt    <  2:70445/81‑1 (MQ=255)                                                                       gTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTAt  >  1:70485/1‑81 (MQ=255)                                                                       gTGGTTGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTAt  >  2:70491/1‑81 (MQ=255)                                                                            |                                                                            AACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTAT  >  NC_000913/304679‑304829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑