Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,738,716	T→C	I324I (ATT→ATC)	pheA →	fused chorismate mutase/prephenate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,738,716	0	T	C	100.0%	93.0 / NA	27	I324I (ATT→ATC)	pheA	fused chorismate mutase/prephenate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/21);  total (6/21)

GTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCA  >  NC_000913/2738644‑2738789                                                                         |                                                                          gttAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGAccc                                                                    <  1:493066/80‑1 (MQ=255)          gACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATc                                                          <  2:493067/81‑1 (MQ=255)            ccGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCAc                                                        <  1:493068/81‑1 (MQ=255)              gggCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGc                                                      >  2:493120/1‑81 (MQ=255)               ggCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGcc                                                     <  1:493069/81‑1 (MQ=255)                     aaGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTc                                               <  2:493070/81‑1 (MQ=255)                          ggTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGt                                          <  2:493071/81‑1 (MQ=255)                          ggTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGt                                          <  2:493072/81‑1 (MQ=255)                           gTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGt                                          >  1:493128/1‑80 (MQ=255)                              cgcTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAAt                                       <  1:493074/80‑1 (MQ=255)                              cgcTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAAt                                       <  2:493073/80‑1 (MQ=255)                               gcTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATcc                                     <  1:493075/81‑1 (MQ=255)                                      gAAGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGa                               <  1:493076/80‑1 (MQ=255)                                       aaGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGaa                              <  2:493078/80‑1 (MQ=255)                                       aaGCGTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAag                             <  2:493077/81‑1 (MQ=255)                                           gTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGAt                         >  2:493135/1‑81 (MQ=255)                                           gTTGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGAt                         <  2:493079/81‑1 (MQ=255)                                            ttGCTGGTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATg                        >  1:493129/1‑81 (MQ=255)                                                 ggTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTa                   >  1:493140/1‑81 (MQ=255)                                                  gTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCt                    <  2:493080/79‑1 (MQ=255)                                                  gTACTGCGCAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTAt                  <  1:493081/81‑1 (MQ=255)                                                          cAACCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGatat          <  2:493082/81‑1 (MQ=255)                                                           aaCCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATAtt         <  2:493083/81‑1 (MQ=255)                                                            aCCACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTc        <  2:493084/81‑1 (MQ=255)                                                             ccACAATCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCa       <  2:493085/81‑1 (MQ=255)                                                                 aaTCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGc    >  1:493086/1‑80 (MQ=255)                                                                  aTCTGATCATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCa  <  1:493087/81‑1 (MQ=255)                                                                         |                                                                          GTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCA  >  NC_000913/2738644‑2738789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑