Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,744,349	A→G	V61A (GTT→GCT)	rplS ←	50S ribosomal subunit protein L19

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,744,349	0	A	G	100.0%	64.1 / NA	20	V61A (GTT→GCT)	rplS	50S ribosomal subunit protein L19
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/12);  total (8/12)

TGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTT  >  NC_000913/2744272‑2744422                                                                              |                                                                          tGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAGCAg                                                                        >  2:494161/1‑81 (MQ=255)         aTGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAGCAGTGAATGCa                                                                <  1:494133/81‑1 (MQ=255)          tGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAGCAGTGAATGCag                                                               <  1:494135/81‑1 (MQ=255)          tGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAGCAGTGAATGCag                                                               <  2:494134/81‑1 (MQ=255)              gTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAGCAGTGAATGCAGAGTg                                                           >  2:494192/1‑81 (MQ=255)               tCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAGCAGTGAATGCAGAGTg                                                           <  1:494136/80‑1 (MQ=255)                           gagtgagtCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAGCAGTGAATGCAGAGTGCAGACCGCGGTTa                                              >  2:494173/1‑81 (MQ=255)                             gtgagtCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAGCAGTGAATGCAGAGTGCAGACCGCGGTTACg                                            <  2:494137/81‑1 (MQ=255)                             gtgagtCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAGCAGTGAATGCAGAGTGCAGACCGCGGTTACg                                            <  2:494138/81‑1 (MQ=255)                                     ggAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAGCAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGa                                    >  1:494172/1‑81 (MQ=255)                                      gAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAGCAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCg                                     <  2:494139/79‑1 (MQ=255)                                          acacGCTCAACGCCTTCGCCGTTGGAAATTTTACGAGCAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAAcc                               <  2:494140/81‑1 (MQ=255)                                                  aaCGCCTTCGCCGTTGGAAATTTTACGAGCAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTc                       <  2:494141/81‑1 (MQ=255)                                                          cGCCGTTGGAAATTTTACGAGCAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCt               >  1:494167/1‑81 (MQ=255)                                                               ttGGAAATTTTACGAGCAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGa          >  1:494170/1‑81 (MQ=255)                                                                  gAAATTTTACGAGCAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGt       >  2:494175/1‑81 (MQ=255)                                                                   aaaTTTTACGAGCAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGtt      <  1:494142/81‑1 (MQ=255)                                                                     aTTTTACGAGCAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGtttt    <  2:494143/81‑1 (MQ=255)                                                                       tttACGAGCAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGtttttt  >  1:494179/1‑81 (MQ=255)                                                                        ttACGAGCAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGtttttt  <  2:494144/80‑1 (MQ=255)                                                                              |                                                                          TGACAGAAATGCTGTCAACTACCGGAGAGTGAGTCTGGAAGACACGCTCAACGCCTTCGCCGTTGGAAATTTTACGAACAGTGAATGCAGAGTGCAGACCGCGGTTACGAATAGCGATAACCACGCCCTCGAATGCCTGCAGACGTTTTTT  >  NC_000913/2744272‑2744422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑