Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,753,649	G→A	M15I (ATG→ATA)	bamE →	outer membrane protein assembly factor BamE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,753,649	0	G	A	100.0%	84.6 / NA	24	M15I (ATG→ATA)	bamE	outer membrane protein assembly factor BamE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/12);  total (12/12)

ACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATGTTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCT  >  NC_000913/2753573‑2753724                                                                             |                                                                            aCTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACtattgatattga                                                                         >  2:496105/1‑81 (MQ=255)     cTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATATTGACCGc                                                                     <  1:496073/81‑1 (MQ=255)                aaaGGAATCAAATCACTATGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATATTGACCGCAGGCTGTTCCa                                                          >  1:496116/1‑81 (MQ=255)                   ggAATCAAATCACTATGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATATTGACCGCAGGCTGTTCCActc                                                       >  1:496104/1‑81 (MQ=255)                    gAATCAAATCACTATGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATATTGACCGCAGGCTGTTCCActct                                                      <  1:496074/81‑1 (MQ=255)                     aatcaaatcaCTATGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATATTGACCGCAGGCTGTTCCACTCTg                                                     >  2:496109/1‑81 (MQ=255)                        caaatcaCTATGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATATTGACCGCAGGCTGTTCCACTCTGGAg                                                  >  2:496119/1‑81 (MQ=255)                              aCTATGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATATTGACCGCAGGCTGTTCCACTCTGGAGCGAGTg                                            <  1:496075/81‑1 (MQ=255)                                 aTGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATATTTACCGCAGGCTGTTCCACTCTGGAGCGAGTGGtt                                         >  2:496118/1‑81 (MQ=255)                                  tGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATATTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGttt                                        <  1:496076/81‑1 (MQ=255)                                        gTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATATTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTc                                  >  2:496111/1‑81 (MQ=255)                                           aaaCGCTGACTGCTGCAGCAGCAGTACTATTGATATTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCt                                <  1:496077/80‑1 (MQ=255)                                            aaCGCTGACTGCTGCAGCAGCAGTACTATTGATATTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGa                              >  1:496117/1‑81 (MQ=255)                                             aCGCTGACTGCTGCAGCAGCAGTACTATTGATATTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGAc                             <  1:496078/81‑1 (MQ=255)                                                   aCTGCTGCAGCAGCAGTACTATTGATATTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCa                         <  2:496079/79‑1 (MQ=255)                                                       ctgcAGCAGCAGTACTATTGATATTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAgg                   >  2:496106/1‑81 (MQ=255)                                                       ctgcAGCAGCAGTACTATTGATATTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAg                    <  2:496080/80‑1 (MQ=255)                                                          cagcagcagTACTATTGATATTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAgggg                 <  2:496081/80‑1 (MQ=255)                                                           agcagcagTACTATTGATATTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGaa               >  1:496110/1‑81 (MQ=255)                                                           agcagcagTACTATTGATATTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGaa               >  2:496121/1‑81 (MQ=255)                                                                cagTACTATTGATATTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATc          <  1:496082/81‑1 (MQ=255)                                                                  gTACTATTGATATTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCt         >  2:496113/1‑80 (MQ=255)                                                                     cTATTGATATTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGAcc     <  2:496083/81‑1 (MQ=255)                                                                        ttGATATTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGc   >  2:496114/1‑80 (MQ=255)                                                                        ttGATATTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCt  <  1:496084/81‑1 (MQ=255)                                                                             |                                                                            ACTGCTCGGGCCCGAAAAGGAATCAAATCACTATGCGCTGTAAAACGCTGACTGCTGCAGCAGCAGTACTATTGATGTTGACCGCAGGCTGTTCCACTCTGGAGCGAGTGGTTTACCGTCCTGACATCAACCAGGGGAACTATCTGACCGCT  >  NC_000913/2753573‑2753724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑