Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,755,418	C→A	intergenic (+40/‑175)	smpB → / → ssrA	SsrA‑binding protein/tmRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,755,418	0	C	A	100.0%	109.7 / NA	31	intergenic (+40/‑175)	smpB/ssrA	SsrA‑binding protein/tmRNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/20);  total (11/20)

GGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTCCCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCATTGCTTAATCG  >  NC_000913/2755342‑2755494                                                                             |                                                                             ggATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGcctacctc                                                                          >  1:496406/1‑81 (MQ=255)   aTAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCtctc                                                                        <  1:496362/81‑1 (MQ=255)    tAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTcc                                                                       <  2:496363/81‑1 (MQ=255)          aCGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCggcggc                                                                 >  2:496432/1‑81 (MQ=255)               tCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGa                                                             <  1:496364/80‑1 (MQ=255)                    aaaaaCGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAAc                                                       <  2:496365/81‑1 (MQ=255)                     aaaaCGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACa                                                      >  2:496421/1‑81 (MQ=255)                       aaCGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATc                                                    >  2:496414/1‑81 (MQ=255)                         cGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCtt                                                  <  2:496366/81‑1 (MQ=255)                         cGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCt                                                   >  2:496419/1‑80 (MQ=255)                               ccGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGc                                            >  2:496410/1‑81 (MQ=255)                                cGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCt                                           <  2:496367/81‑1 (MQ=255)                                  ttAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTAt                                         <  2:496368/81‑1 (MQ=255)                                    aaaCCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATc                                        <  2:496369/80‑1 (MQ=255)                                     aaCCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATcac                                      <  1:496370/81‑1 (MQ=255)                                        cTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATc                                   >  1:496411/1‑81 (MQ=255)                                           cACTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATcc                                  <  2:496371/79‑1 (MQ=255)                                            aCTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGac                               <  2:496372/81‑1 (MQ=255)                                             cTCCAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGaca                              >  2:496412/1‑81 (MQ=255)                                                cAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGacac                             <  2:496374/79‑1 (MQ=255)                                                cAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACaa                           <  2:496373/81‑1 (MQ=255)                                                cAATTATTGACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACaa                           <  2:496375/81‑1 (MQ=255)                                                        gACCAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGc                    >  2:496426/1‑80 (MQ=255)                                                           cAGTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATc                <  2:496376/81‑1 (MQ=255)                                                             gTTCCTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATcc               >  2:496423/1‑80 (MQ=255)                                                                 cTCACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCAtt           <  2:496377/80‑1 (MQ=255)                                                                   cACCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCATTGCt        >  1:496446/1‑81 (MQ=255)                                                                    aCCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCATTGc         <  2:496378/79‑1 (MQ=255)                                                                    aCCGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCATTGCtt       <  2:496379/81‑1 (MQ=255)                                                                     ccGCGCCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCATTGCtt       >  2:496425/1‑80 (MQ=255)                                                                        cgcCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCATTGCTTAATc   <  2:496380/81‑1 (MQ=255)                                                                        cgcCTACCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCATTGCTTAATc   <  1:496381/81‑1 (MQ=255)                                                                          cctacctcTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCATTGCTTAATCg  >  2:496416/1‑80 (MQ=255)                                                                             |                                                                             GGATAAAGCACGTATCATGAAAAACGCCCACCGTTAAACCTGCACTCCAATTATTGACCAGTTCCTCACCGCGCCTCCCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCATTGCTTAATCG  >  NC_000913/2755342‑2755494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑