Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,780,268	T→C	Q820R (CAA→CGA)	ypjA ←	adhesin‑like autotransporter YpjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,780,268	0	T	C	100.0%	167.6 / NA	48	Q820R (CAA→CGA)	ypjA	adhesin‑like autotransporter YpjA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/22);  total (26/22)

TCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGAC  >  NC_000913/2780198‑2780341                                                                       |                                                                          tcCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCAccg                                                                   <  1:500631/79‑1 (MQ=255)   cGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCAccgcc                                                                 <  1:500632/79‑1 (MQ=255)   cGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCgg                                                               <  2:500634/81‑1 (MQ=255)   cGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCgg                                                               <  2:500633/81‑1 (MQ=255)     ttAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGt                                                              >  1:500691/1‑80 (MQ=255)       aGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAg                                                           >  1:500679/1‑81 (MQ=255)       aGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAg                                                           >  2:500693/1‑81 (MQ=255)        gCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGc                                                          <  1:500635/81‑1 (MQ=255)           gAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGcgtc                                                       >  2:500688/1‑81 (MQ=255)               cTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGc                                                   <  2:500636/81‑1 (MQ=255)               cTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGc                                                   >  2:500684/1‑81 (MQ=255)                  tttGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGcc                                                  <  1:500638/79‑1 (MQ=255)                  tttGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCt                                                 <  1:500637/80‑1 (MQ=255)                  tttGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTg                                                >  1:500700/1‑81 (MQ=255)                     gTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTc                                              <  1:500640/80‑1 (MQ=255)                     gTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTc                                              <  2:500639/80‑1 (MQ=255)                      tATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCg                                             >  1:500690/1‑80 (MQ=255)                      tATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGc                                            <  1:500641/81‑1 (MQ=255)                        ttCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAg                                          >  2:500685/1‑81 (MQ=255)                        ttCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAg                                          <  1:500642/81‑1 (MQ=255)                         tCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGt                                         >  2:500697/1‑81 (MQ=255)                           cgccgTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTg                                        <  1:500643/80‑1 (MQ=255)                            gccgTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGc                                      >  2:500692/1‑81 (MQ=255)                              cgtcgtTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCtt                                    >  2:500694/1‑81 (MQ=255)                               gtcgtTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGGGCCTGTCGCAGTGGCttt                                   <  2:500644/81‑1 (MQ=255)                                tcgtTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCtttt                                  <  1:500645/81‑1 (MQ=255)                                 cgtTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCttttg                                 >  1:500722/1‑81 (MQ=255)                                  gtTAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCttttg                                 <  2:500646/80‑1 (MQ=255)                                    tAATTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCttttgttt                              >  1:500698/1‑81 (MQ=255)                                     aaTTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCttttgtttt                             >  1:500705/1‑81 (MQ=255)                                     aaTTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCCGTCGCAGTGGCttttgtttt                             >  1:500701/1‑81 (MQ=255)                                      aTTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCttttgttttg                            <  2:500647/81‑1 (MQ=255)                                      aTTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCttttgtttt                             >  1:500711/1‑80 (MQ=255)                                      aTTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCttttgtttt                             >  2:500696/1‑80 (MQ=255)                                      aTTGTTGTCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCttttgtttt                             >  1:500686/1‑80 (MQ=255)                                          ttgtCTCATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGa                        <  2:500648/81‑1 (MQ=255)                                              ctcATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGAtctc                    >  2:500704/1‑81 (MQ=255)                                                cATTCGCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTccc                  >  2:500709/1‑81 (MQ=255)                                                     gCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTcc              <  1:500649/80‑1 (MQ=255)                                                     gCCACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCg             >  1:500713/1‑81 (MQ=255)                                                      ccACACCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGc            <  2:500650/81‑1 (MQ=255)                                                        acacCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGcc           >  1:500689/1‑80 (MQ=255)                                                          acCCTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCa         >  2:500702/1‑80 (MQ=255)                                                             cTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATAtt     <  1:500652/81‑1 (MQ=255)                                                             cTCGACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATAtt     <  2:500651/81‑1 (MQ=255)                                                                gACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGAc  >  1:500714/1‑81 (MQ=255)                                                                gACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGAc  >  2:500712/1‑81 (MQ=255)                                                                gACAATTCGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGAc  >  2:500724/1‑81 (MQ=255)                                                                       |                                                                          TCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGAC  >  NC_000913/2780198‑2780341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑