Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,786,329	T→C	intergenic (‑492/‑68)	ileY ← / → ygaQ	tRNA‑Ile/putative uncharacterized protein YgaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,786,329	0	T	C	100.0%	28.9 / NA	10	intergenic (‑492/‑68)	ileY/ygaQ	tRNA‑Ile/putative uncharacterized protein YgaQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/5);  total (5/5)

ACAGATTTAAGCATTATCCTTTTCCATATAAATATTGGATAAAAGTAGGACATCTGTTTGCAATTACTTTCACAACAATTAAACATTTTTATGTTTCCGTATACATCATATTACTCTACCATTAGAGGAACTTTATTATGTT  >  NC_000913/2786260‑2786401                                                                      |                                                                         aCAGATTTGAGCATTATCCTTTTCTATATAAATATTGGATAAAAGTAGGACATCTGTTTGCAATTACTTCCACAACAAtt                                                                >  1:501569/1‑80 (MQ=255)                      ttCCATATAAATATTGGATAAAAGTAGGACATCTGTTTGCAATTACTTCCACAACAATTAAACATTTTTATGTTTCCGt                                            <  1:501556/79‑1 (MQ=255)                      ttCCATATAAATATTGGATAAAAGTAGGACATCTGTTTGCAATTACTTCCACAACAATTAAACATTTTTATCTTTCCGta                                           <  1:501557/80‑1 (MQ=255)                              aaaTATTGGATAAAAGTAGGACATCTGTTTGCAATTACTTCCACAACAATTAAACATTTTTATGTTTCCGTATACATCata                                  >  2:501572/1‑81 (MQ=255)                                    tGGATAAAAGTAGGACATCTGTTTGCAATTACTTCCACAACAATTAAACATTTTTATGTTTCCGTATACATCATATTActc                            >  2:501574/1‑81 (MQ=255)                                          aaaGTAGGACATCTGTTTGCAATTACTTCCACAACAATTAAACATTTTTATGTTTCCGTATACATCATATTACTCTACCAt                      <  2:501558/81‑1 (MQ=255)                                           aaGTAGGACATCTGTTTGCAATTACTTCCACAACAATTAAACATTTTTATGTTTCCGTATACATCATATTACTCTACCAt                      >  2:501570/1‑80 (MQ=255)                                                    aTCTGTTTGCAATTACTTCCACAACAATTAAACATTTTTATGTTTCCGTATACATCATATTACTCTACCATTAGAGGAACt            <  2:501559/81‑1 (MQ=255)                                                         tttGCAATTACTTCCACAACAATTAAACATTTTTATGTTTCCGTATACATCATATTACTCTACCATTAGAGGAACTttat        >  2:501579/1‑80 (MQ=255)                                                              aaTTACTTCCACAACAATTAAACATTTTTATGTTTCCGTATACATCATATTACTCTACCATTAGAGGAACTTTATTATGtt  <  2:501560/81‑1 (MQ=255)                                                                      |                                                                         ACAGATTTAAGCATTATCCTTTTCCATATAAATATTGGATAAAAGTAGGACATCTGTTTGCAATTACTTTCACAACAATTAAACATTTTTATGTTTCCGTATACATCATATTACTCTACCATTAGAGGAACTTTATTATGTT  >  NC_000913/2786260‑2786401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑