Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,796,579	T→C	I70V (ATC→GTC)	kbp ←	K(+) binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,796,579	0	T	C	100.0%	94.8 / NA	28	I70V (ATC→GTC)	kbp	K(+) binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/10);  total (18/10)

GGCAGTGGCTGGTGTCGCCGTTTTCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGATTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAATATTCACTT  >  NC_000913/2796502‑2796653                                                                              |                                                                           ggcAGTGGCTGGTGTCGCCGTTTTCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGActtt                                                                         >  1:503588/1‑81 (MQ=255)     gTGGCTGGTGTCGCCGTTTTCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTcc                                                                     >  2:503591/1‑81 (MQ=255)     gTGGCTGGTGTCGCCGTTTTCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTcc                                                                     >  1:503600/1‑81 (MQ=255)            gtgtCGCCGTTTTCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGcct                                                              >  2:503594/1‑81 (MQ=255)            gtgtCGCCGTTTTCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGcc                                                               <  2:503545/80‑1 (MQ=255)                 gCCGTTTTCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTg                                                          <  2:503546/80‑1 (MQ=255)                       ttCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAgg                                                   >  2:503589/1‑81 (MQ=255)                        tCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGc                                                  >  2:503590/1‑81 (MQ=255)                          aCCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGca                                                 <  2:503547/80‑2 (MQ=255)                           ccTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGt                                               >  2:503605/1‑81 (MQ=255)                                   tcGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTg                                       <  1:503548/81‑1 (MQ=255)                                   tcGACACTGGCAATACCGGAAATAATCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTg                                       <  1:503549/81‑1 (MQ=255)                                      acacTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGAcc                                    <  2:503550/81‑1 (MQ=255)                                      acacTGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGAcc                                    >  1:503601/1‑81 (MQ=255)                                          tGGCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCg                                >  1:503583/1‑81 (MQ=255)                                            gCAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCt                              <  2:503551/81‑1 (MQ=255)                                             cAATACCGGAAATATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCtt                             >  2:503593/1‑81 (MQ=255)                                                      aaaTATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCgg                    >  2:503603/1‑81 (MQ=255)                                                      aaaTATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCgg                    >  2:503607/1‑81 (MQ=255)                                                       aaTATTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCgg                    >  2:503606/1‑80 (MQ=255)                                                         tatTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCaa                 <  2:503552/81‑1 (MQ=255)                                                          atTCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAAt                >  1:503602/1‑81 (MQ=255)                                                           ttCCCCACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAAtt               <  1:503553/81‑1 (MQ=255)                                                              cccACCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTg             <  1:503554/80‑1 (MQ=255)                                                                 aCCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAata         >  1:503595/1‑81 (MQ=255)                                                                 aCCGCAACAAGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAata         >  2:503622/1‑81 (MQ=255)                                                                     caacaaGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAATATTCa     >  2:503609/1‑81 (MQ=255)                                                                      aacaaGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAATATTCAc    >  2:503586/1‑81 (MQ=255)                                                                         aaGGACTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAATATTCACtt  >  1:503599/1‑80 (MQ=255)                                                                              |                                                                           GGCAGTGGCTGGTGTCGCCGTTTTCACCTGATCATCGACACTGGCAATACCGGAAATATTCCCCACCGCAACAAGGATTTTCTCCTTCGCCTCCTGACTCAGGCCGTCACCAGTGACCGTCGCTTTGCCGTCGGCAATTTGAATATTCACTT  >  NC_000913/2796502‑2796653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑