Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,797,709	T→C	C64R (TGC→CGC)	ygaP →	thiosulfate sulfurtransferase YgaP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,797,709	0	T	C	100.0%	104.9 / NA	31	C64R (TGC→CGC)	ygaP	thiosulfate sulfurtransferase YgaP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/11);  total (20/11)

TCTGGCTCCATTATCCGTGCTGGAACAGTCAGGTCTTCCGGCTAAATTACGTCACGAGCAAATTATATTCCACTGCCAGGCAGGTAAACGCACCAGTAATAATGCCGATAAATTAGCAGCGATTGCCGCCCCCGCAGAAATCTTTTTACT  >  NC_000913/2797636‑2797785                                                                          |                                                                             tCTGGCTCCATTATCCGTGCTGGAACAGTCAGGTCTTCCGGCTAAATTACGTCACGAGCAAATTATATTCCACCGCcagg                                                                        >  1:503799/1‑80 (MQ=255)    ggCTCCATTATCCGTGCTGGAACAGTCAGGTCTTCCGGCTAAATTACGTCACGAGCAAATTATATTCCACCGCcaggcagg                                                                    >  2:503785/1‑81 (MQ=255)     gCTCCATTATCCGTGCTGGAACAGTCAGGTCTTCCGGCTAAATTACGTCACGAGCAAATTATATTCCACCGCcaggcagg                                                                    >  2:503782/1‑80 (MQ=255)         cATTATCCGTGCTGGAACAGTCAGGTCTTCCGGCTAAATTACGTCACGAGCAAATTATATTCCACCGCCAGGCAGGTAAAc                                                               <  2:503757/81‑1 (MQ=255)           ttATCCGTGCTGGAACAGTCAGGTCTTCCGGCTAAATTACGTCACGAGCAAATTATATTCCACCGCCAGGCAGGTAAACGc                                                             <  2:503758/81‑1 (MQ=255)             aTCCGTGCTGGAACAGTCAGGTCTTCCGGCTAAATTACGTCACGAGCAAATTATATTCCACCGCCAGGCAGGTAAACGCAc                                                           >  1:503784/1‑81 (MQ=255)                 gTGCTGGAACAGTCAGGTCTTCCGGCTAAATTACGTCACGAGCAAATTATATTCCACCGCCAGGCAGGTAAACGCACCAGt                                                       <  2:503759/81‑1 (MQ=255)                    cTGGAACAGTCAGGTCTTCCGGCTAAATTACGTCACGAGCAAATTATATTCCACCGCCAGGCAGGTAAACGCACCAGtaat                                                    <  2:503760/81‑1 (MQ=255)                    cTGGAACAGTCAGGTCTTCCGGCTAAATTACGTCACGAGCAAATTATATTCCACCGCCAGGCAGGTAAACGCACCAGtaa                                                     <  1:503761/80‑1 (MQ=255)                      ggAACAGTCAGGTCTTCCGGCTAAATTACGTCACGAGCAAATTATATTCCACCGCCAGGCAGGTAAACGCACCAGtaataa                                                  >  2:503791/1‑81 (MQ=255)                           aGTCAGGTCTTCCGGCTAAATTACGTCACGAGCAAATTATATTCCACCGCCAGGCAGGTAAACGCACCAGTAATAATGcc                                              >  1:503824/1‑80 (MQ=255)                           aGTCAGGTATTCCGGCTAAATTACGTCACGAGCAAATTATATTCCACCGCCAGGCAGGTAAACGCACCAGTAATAATGcc                                              >  2:503788/1‑80 (MQ=255)                               aGGTCTTCCGGCTAAATTACGTCACGAGCAAATTATATTCCACCGCCAGGCAGGTAAACGCACCAGTAATAATGCCGATa                                          >  1:503794/1‑80 (MQ=255)                               aGGTCTTCCGGCTAAATTACGTCACGAGCAAATTATATTCCACCGCCAGGCAGGTAAACGCACCAGTAATAATGCCGATa                                          >  1:503827/1‑80 (MQ=255)                                ggTCTTCCGGCTAAATTACGTCACGAGCAAATTATATTCCACCGCCAGGCAGGTAAACGCACCAGTAATAATGCCGATaaa                                        >  1:503801/1‑81 (MQ=255)                                 gTCTTCCGGCTAAATTACGTCACGAGCAAATTATATTCCACCGCCAGGCAGGTAAACGCACCAGTAATAATGCCGATAAAt                                       >  2:503786/1‑81 (MQ=255)                                     tCCGGCTAAATTACGTCACGAGCAAATTATATTCCACCGCCAGGCAGGTAAACGCACCAGTAATAATGCCGATAAATTag                                    >  1:503792/1‑80 (MQ=255)                                     tCCGGCTAAATTACGTCACGAGCAAATTATATTCCACCGCCAGGCAGGTAAACGCACCAGTAATAATGCCGATAAATTag                                    >  1:503804/1‑80 (MQ=255)                                      ccGGCTAAATTACGTCACGAGCAAATTATATTCCACCGCCAGGCAGGTAAACGCACCAGTAATAATGCCGATAAATTag                                    >  1:503787/1‑79 (MQ=255)                                        ggCTAAATTACGTCACGAGCAAATTATATTCCACCGCCAGGCAGGTAAACGCACCAGTAATAATGCCGATAAATTagcagc                                >  1:503793/1‑81 (MQ=255)                                          cTAAATTACGTCACGAGCAAATTATATTCCACCGCCAGGCAGGTAAACGCACCAGTAATAATGCCGATAAATTAGCAGCGa                              <  1:503762/81‑1 (MQ=255)                                             aaTTACGTCACGAGCAAATTATATTCCACCGCCAGGCAGGTAAACGCACCAGTAATAATGCCGATAAATTAGCAGCGAtt                            <  2:503763/80‑1 (MQ=255)                                                   gTCACGAGCAAATTATATTCCACCGCCAGGCAGGTAAACGCACCAGTAATAATGCCGATAAATTAGCAGCGATTGCCGccc                     >  2:503805/1‑81 (MQ=255)                                                      aCGAGCAAATTATATTCCACCGCCAGGCAGGTAAACGCACCAGTAATAATGCCGATAAATTAGCAGCGATTGCCGccccc                   <  1:503764/80‑1 (MQ=255)                                                         aGCAAATTATATTCCACCGCCAGGCAGGTAAACGCACCAGTAATAATGCCGATAAATTAGCAGCGATTGCCGCCCCCGCAg               <  1:503766/81‑1 (MQ=255)                                                         aGCAAATTATATTCCACCGCCAGGCAGGTAAACGCACCAGTAATAATGCCGATAAATTAGCAGCGATTGCCGCCCCCGCAg               <  1:503765/81‑1 (MQ=255)                                                             aaTTATATTCCACCGCCAGGCAGGTAAACGCACCAGTAATAATGCCGATAAATTAGCAGCGATTGCCGCCCCCGCAGAAAt           >  1:503806/1‑81 (MQ=255)                                                             aaTTATATTCCACCGCCAGGCAGGTAAACGCACCAGTAATAATGCCGATAAATTAGCAGCGATTGCCGCCCCCGCAGAAAt           >  2:503790/1‑81 (MQ=255)                                                             aaTTATATTCCACCGCCAGGCAGGTAAACGCACCAGTAATAATGCCGATAAATTAGCAGCGATTGCCGCCCCCGCAGAAAt           >  2:503800/1‑81 (MQ=255)                                                                     tCCACCGCCAGGCAGGTAAACGCACCAGTAATAATGCCGATAAATTAGCAGCGATTGCCGCCCCCGCAGAAATCttttttc   >  2:503797/1‑79 (MQ=255)                                                                       cACCGCCAGGCAGGTAAACGCACCAGTAATAATGCCGATAAATTAGCAGCGATTGCCGCCCCCGCAGAAATCTTTTTACt  <  1:503767/80‑1 (MQ=255)                                                                          |                                                                             TCTGGCTCCATTATCCGTGCTGGAACAGTCAGGTCTTCCGGCTAAATTACGTCACGAGCAAATTATATTCCACTGCCAGGCAGGTAAACGCACCAGTAATAATGCCGATAAATTAGCAGCGATTGCCGCCCCCGCAGAAATCTTTTTACT  >  NC_000913/2797636‑2797785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑