Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,806,414	C→T	A135A (GCC→GCT)	proW →	glycine betaine ABC transporter membrane subunit ProW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,806,414	0	C	T	100.0%	132.6 / NA	37	A135A (GCC→GCT)	proW	glycine betaine ABC transporter membrane subunit ProW
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/23);  total (14/23)

TCGTTTTCGCTCTCATCGCCTGGCAGATTTCCGGGGTCGGAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCG  >  NC_000913/2806341‑2806490                                                                          |                                                                             tCGTTTTCGCTCTCATCGCCTGGCAGATTTCCGGGGTCGGAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCTATCGgc                                                                        <  1:505464/80‑1 (MQ=255)      ttCGCTCTCATCGCCTGGCAGATTTCCGGGGTCGGAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAAt                                                                   <  1:505465/80‑1 (MQ=255)       tCGCTCTCATCGCCTGGCAGATTTCCGGGGTCGGAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCg                                                                 >  2:505524/1‑81 (MQ=255)         gCTCTCATCGCCTGGCAGATTTCCGGGGTCGGAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCgg                                                                <  2:505466/80‑1 (MQ=255)         gCTCTCATCGCCTGGCAGATTTCCGGGGTCGGAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCGGt                                                               <  1:505467/81‑1 (MQ=255)         gCTCTCATCGCCTGGCAGATTTCCGGGGTCGGAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCGGt                                                               <  1:505468/81‑1 (MQ=255)              cATCGCCTGGCAGATTTCCGGGGTCGGAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTg                                                          >  1:505512/1‑81 (MQ=255)               aTCGCCTGGCAGATTTCCGGGGTCGGAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTgg                                                         <  2:505470/81‑1 (MQ=255)               aTCGCCTGGCAGATTTCCGGGGTCGGAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTgg                                                         <  1:505469/81‑1 (MQ=255)               aTCGCCTGGCAGATTTCCGGGGTCGGAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTgg                                                         <  1:505471/81‑1 (MQ=255)                tCGCCTGGCAGATTTCCGGGGTCGGAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTGGt                                                        >  1:505521/1‑81 (MQ=255)                  gCCTGGCAGATTTCCGGGGTCGGAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTGGTCg                                                      <  1:505472/81‑1 (MQ=255)                   ccTGGCAGATTTCCGGGGTCGGAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTGGTCGc                                                     <  2:505473/81‑1 (MQ=255)                     tGGCAGATTTCCGGGGTCGGAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTGGTCGCAg                                                   >  1:505530/1‑81 (MQ=255)                      ggCAGATTTCCGGGGTCGGAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTGGTCGCAgg                                                  >  1:505518/1‑81 (MQ=255)                              tCCGGGGTCGGAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGt                                           >  1:505519/1‑80 (MQ=255)                                cGGGGTCGGAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGAc                                        >  1:505526/1‑81 (MQ=255)                                     tCGGAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTgg                                   >  2:505527/1‑81 (MQ=255)                                       ggAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGgcg                                 <  1:505474/81‑1 (MQ=255)                                        gAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGgcgc                                <  1:505475/81‑1 (MQ=255)                                              gtgtGGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGgtgt                          <  2:505476/81‑1 (MQ=255)                                               tgtgGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGtt                         <  2:505477/81‑1 (MQ=255)                                                 tgGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTa                        <  1:505479/80‑1 (MQ=255)                                                 tgGCGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTa                        <  2:505478/80‑1 (MQ=255)                                                    cGACGCTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCg                    >  2:505528/1‑81 (MQ=255)                                                        gCTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCct                >  1:505509/1‑81 (MQ=255)                                                        gCTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCct                <  1:505480/81‑1 (MQ=255)                                                         cTGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCctg               >  2:505529/1‑81 (MQ=255)                                                          tGGTTTCGCTGATTGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCctgc              >  1:505523/1‑81 (MQ=255)                                                             tttCGCTGATTGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGt           <  2:505481/81‑1 (MQ=255)                                                              ttCGCTGATTGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGtt          >  2:505516/1‑81 (MQ=255)                                                               tCGCTGATTGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGtt          <  2:505482/80‑1 (MQ=255)                                                                  cTGATTGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTg       >  1:505533/1‑80 (MQ=255)                                                                  cTGATTGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGt      <  1:505483/81‑1 (MQ=255)                                                                     aTTGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATc   <  2:505484/81‑1 (MQ=255)                                                                     aTTGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATc   <  2:505485/81‑1 (MQ=255)                                                                      ttGCTATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCg  <  1:505486/81‑1 (MQ=255)                                                                          |                                                                             TCGTTTTCGCTCTCATCGCCTGGCAGATTTCCGGGGTCGGAATGGGTGTGGCGACGCTGGTTTCGCTGATTGCCATCGGCGCAATCGGTGCCTGGTCGCAGGCAATGGTGACTCTGGCGCTGGTGTTAACCGCCCTGCTGTTCTGTATCG  >  NC_000913/2806341‑2806490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑