Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,814,420	T→C	K105R (AAA→AGA)	luxS ←	S‑ribosylhomocysteine lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,814,420	0	T	C	100.0%	88.2 / NA	26	K105R (AAA→AGA)	luxS	S‑ribosylhomocysteine lyase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/12);  total (14/12)

AGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCAATCAGACTCATATAAAAACCGGTGCGGCAGCCCATT  >  NC_000913/2814344‑2814494                                                                             |                                                                           aGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTCTCCa                                                                        <  2:507268/81‑1 (MQ=255) aGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTCTCCa                                                                        <  1:507267/81‑1 (MQ=255)   tGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTCTCCAgg                                                                      >  2:507316/1‑81 (MQ=255)         acTGGTAGACGTTCAGTTCCGGGATCTGATTCTTATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTCTCCAGGCATCAg                                                                <  1:507269/81‑1 (MQ=255)           tGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTCTCCAGGCATCAGc                                                               >  1:507310/1‑80 (MQ=255)             gTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTCTCCAGGCATCAGCaa                                                             <  2:507270/80‑1 (MQ=255)             gTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTCTCCAGGCATCAGCAac                                                            <  2:507271/81‑1 (MQ=255)                gACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTCTCCAGGCATCAGCAacac                                                          <  1:507274/80‑1 (MQ=255)                gACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTCTCCAGGCATCAGCAacac                                                          <  2:507272/80‑1 (MQ=255)                gACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTCTCCAGGCATCAGCAACACg                                                         >  2:507305/1‑81 (MQ=255)                gACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTCTACAGGCATCAGCAacac                                                          <  2:507273/80‑1 (MQ=255)                       aGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTCTCCAGGCATCAGCAACACGCTGCTCa                                                  >  1:507313/1‑81 (MQ=255)                       aGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTCTCCAGGCATCAGCAACACGCTGCTCa                                                  <  1:507275/81‑1 (MQ=255)                          tCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTCTCCAGGCATCAGCAACACGCTGCTCATCt                                               >  1:507306/1‑81 (MQ=255)                          tCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTCTCCAGGCATCAGCAACACGCTGCTCATCt                                               >  2:507312/1‑81 (MQ=255)                           ccGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTCTCCAGGCATCAGCAACACGCTGCTCATCTg                                              >  1:507299/1‑81 (MQ=255)                                 tctgattctgatCCTGCACTTTCAGCACGTCTTCCATTGCCGCTCTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTAc                                        >  2:507300/1‑81 (MQ=255)                                    gattctgatCCTGCACTTTCAGCACGTCTTCCATTGCCGCTCTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCaa                                     >  1:507309/1‑81 (MQ=255)                                        ctgatCCTGCACTTTCAGCACGTCTTCCATTGCCGCTCTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCAAt                                    >  1:507311/1‑78 (MQ=255)                                          gatCCTGCACTTTCAGCACGTCTTCCATTGCCGCTCTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCAATCAGAc                               >  1:507318/1‑81 (MQ=255)                                           atCCTGCACTTTCAGCACGTCTTCCATTGCCGCTCTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCAATCAGAc                               <  1:507276/80‑1 (MQ=255)                                                          cACGTCTTCCATTGCCGCTCTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCAATCAGACTCATATAAAAACCGGt               >  1:507317/1‑81 (MQ=255)                                                            cGTCTTCCATTGCCGCTCTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCAATCAGACTCATATAAAAACCGGTGc             <  1:507277/81‑1 (MQ=255)                                                             gTCTTCCATTGCCGCTCTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCAATCAGACTCATATAAAAACCGGTGCg            <  1:507278/81‑1 (MQ=255)                                                                   cATTGCCGCTCTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCAATCAGACTCATATAAAAACCGGTGCGGCAGcc      >  2:507319/1‑81 (MQ=255)                                                                       gCCGCTCTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCAATCAGACTCATATAAAAACCGGTGCGGCAGCCCAtt  >  2:507304/1‑81 (MQ=255)                                                                             |                                                                           AGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCAATCAGACTCATATAAAAACCGGTGCGGCAGCCCATT  >  NC_000913/2814344‑2814494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑