Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,854,148	G→A	D299N (GAC→AAC)	hypE →	hydrogenase maturation protein, carbamoyl dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,854,148	0	G	A	100.0%	91.3 / NA	26	D299N (GAC→AAC)	hypE	hydrogenase maturation protein, carbamoyl dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/14);  total (12/14)

AACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTC  >  NC_000913/2854074‑2854225                                                                           |                                                                              aaCTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAAACgcgg                                                                         <  2:516766/81‑1 (MQ=255)      gTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCt                                                                     <  2:516767/80‑1 (MQ=255)       tAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGa                                                                   <  1:516768/81‑1 (MQ=255)        aaTAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGAt                                                                  >  1:516821/1‑81 (MQ=255)          tAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGATTg                                                                >  2:516835/1‑81 (MQ=255)           aGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGATTgg                                                               >  2:516813/1‑81 (MQ=255)                ttGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGATTGGTGaa                                                           <  2:516770/80‑1 (MQ=255)                ttGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGATTGGTGAAg                                                          >  2:516818/1‑81 (MQ=255)                ttGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGATTGGTGAAg                                                          <  2:516769/81‑1 (MQ=255)                      gcaacgcGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGATTGGTGAAGtggtgg                                                    <  1:516772/81‑1 (MQ=255)                      gcaacgcGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGATTGGTGAAGtggtgg                                                    <  2:516771/81‑1 (MQ=255)                        aacgcGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGATTGGTGAAGTGGTGGaa                                                  >  1:516838/1‑81 (MQ=255)                        aacgcGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGATTGGTGAAGTGGTGGaa                                                  >  2:516825/1‑81 (MQ=255)                        aacgcGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGATTGGTGAAGTGGTGGaa                                                  <  2:516773/81‑1 (MQ=255)                         acgcGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGATTGGTGAAGTGGTGGAAc                                                 >  2:516828/1‑81 (MQ=255)                           gcgGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGATTGGTGAAGTGGTGGAACg                                                >  1:516832/1‑80 (MQ=255)                           gcgGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGATTGGTGAAGTGGTGGAACGt                                               <  1:516774/81‑1 (MQ=255)                             ggCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGATTGGTGAAGTGGTGGAACGTa                                              >  2:516841/1‑80 (MQ=255)                             ggCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGATTGGTGAAGTGGTGGAACGTa                                              >  1:516822/1‑80 (MQ=255)                                  agCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGgtg                                        >  2:516834/1‑81 (MQ=255)                                    cAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGtt                                      <  2:516775/81‑1 (MQ=255)                                            ggCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGc                              >  2:516827/1‑81 (MQ=255)                                             gCAGCGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGc                              <  1:516776/80‑1 (MQ=255)                                                 cGTTACATTCCCATCCACTGGGGAAAAACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTc                         <  2:516777/81‑1 (MQ=255)                                                                     gggAAAAACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAAcc     <  1:516778/81‑1 (MQ=255)                                                                       gAAAAACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCt   <  1:516779/81‑1 (MQ=255)                                                                        aaaaACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTc  <  1:516780/81‑1 (MQ=255)                                                                        aaaaACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTc  >  1:516845/1‑81 (MQ=255)                                                                           |                                                                              AACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTC  >  NC_000913/2854074‑2854225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑