Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,858,782	A→C	T564P (ACC→CCC)	mutS →	DNA mismatch repair protein MutS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,858,782	0	A	C	100.0%	90.9 / NA	27	T564P (ACC→CCC)	mutS	DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/13);  total (14/13)

GGAAGCGTTGCAACAGAGCGCGAGCGCGCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATACCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGA  >  NC_000913/2858709‑2858858                                                                          |                                                                             ggAAGCGTTGCAACAGAGCGCGAGCGCGCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGaa                                                                       >  2:517873/1‑81 (MQ=255)   aaGCGTTGCAACAGAGCGCGAGCGCGCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAAc                                                                      >  2:517894/1‑80 (MQ=255)            aaCAGAGCGCGAGCGCGCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGTGCCTATCCCCTGAACTACACCTGcc                                                            <  2:517835/81‑1 (MQ=255)              cAGAGCGCGAGCGCGCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCg                                                          <  1:517836/81‑1 (MQ=255)                 agcgcgagcgcgCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGAcc                                                       <  1:517837/81‑1 (MQ=255)                 agcgcgagcgcgCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGAcc                                                       >  1:517893/1‑81 (MQ=255)                   cgcgagcgcgCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCtt                                                     <  2:517838/81‑1 (MQ=255)                     cgagcgcgCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCa                                                   <  2:517839/81‑1 (MQ=255)                          gcgcTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGAt                                              <  1:517840/81‑1 (MQ=255)                            gcTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATa                                             >  2:517889/1‑80 (MQ=255)                             cTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATaaa                                           >  1:517878/1‑81 (MQ=255)                               ggCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAAcc                                         >  2:517890/1‑81 (MQ=255)                               ggCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAAcc                                         >  1:517887/1‑81 (MQ=255)                                gCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAAc                                          <  2:517841/79‑1 (MQ=255)                                  ggAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCggg                                      >  2:517908/1‑81 (MQ=255)                                  ggAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCggg                                      <  2:517842/81‑1 (MQ=255)                                    aaCTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCa                                    >  2:517886/1‑81 (MQ=255)                                         gACGTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGc                               <  2:517843/81‑1 (MQ=255)                                            gTGCTGGTTAACCTGGCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCAt                             >  1:517909/1‑80 (MQ=255)                                                          ggCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCa              >  2:517891/1‑81 (MQ=255)                                                          ggCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCa              >  1:517900/1‑81 (MQ=255)                                                           gCGGAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCAt             <  2:517844/81‑1 (MQ=255)                                                             ggAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATcc           <  1:517845/81‑1 (MQ=255)                                                             ggAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATcc           <  1:517846/81‑1 (MQ=255)                                                             ggAACGGGCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATcc           >  1:517898/1‑81 (MQ=255)                                                                  gggCCTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAg      >  1:517906/1‑81 (MQ=255)                                                                      cTATCCCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGa  <  2:517847/81‑1 (MQ=255)                                                                          |                                                                             GGAAGCGTTGCAACAGAGCGCGAGCGCGCTGGCGGAACTCGACGTGCTGGTTAACCTGGCGGAACGGGCCTATACCCTGAACTACACCTGCCCGACCTTCATTGATAAACCGGGCATTCGCATTACCGAAGGTCGCCATCCGGTAGTTGA  >  NC_000913/2858709‑2858858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑