Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,865,111	T→C	I4T (ATT→ACT)	ygbN →	putative transporter YgbN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,865,111	0	T	C	100.0%	111.1 / NA	32	I4T (ATT→ACT)	ygbN	putative transporter YgbN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/20);  total (12/20)

AACGTACCCATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCATTC  >  NC_000913/2865033‑2865187                                                                               |                                                                             aaCGTACCCATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTa                                                                            >  1:519225/1‑81 (MQ=255)   cGTACCCATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACa                                                                          <  2:519197/81‑1 (MQ=255)    gTACCCATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACAt                                                                         >  1:519241/1‑81 (MQ=255)     tACCCATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACAtt                                                                        >  2:519238/1‑81 (MQ=255)         cATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTa                                                                    <  2:519198/81‑1 (MQ=255)           tACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATg                                                                  <  2:519199/81‑1 (MQ=255)                ccccccATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTg                                                             >  1:519250/1‑81 (MQ=255)                 cccccATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGc                                                            <  1:519200/81‑1 (MQ=255)                           cGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCt                                                     <  1:519201/78‑1 (MQ=255)                              aTGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTa                                               <  2:519202/81‑1 (MQ=255)                                 gggTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATc                                            <  1:519203/81‑1 (MQ=255)                                  gttAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCa                                           >  1:519258/3‑81 (MQ=255)                                  ggTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCa                                           >  1:519253/1‑81 (MQ=255)                                    tAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATg                                         >  2:519252/1‑81 (MQ=255)                                        ttAACTATAACTTTCAGACAGGGTTTCCCATATCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGt                                     <  2:519204/81‑1 (MQ=255)                                          aaCTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGtt                                    <  2:519205/80‑1 (MQ=255)                                           aCTATAACTTTCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTtgc                                  <  1:519206/81‑1 (MQ=255)                                                 aCTTTCAGACAGGGTTTCCCATGTCTACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGctgctg                             <  2:519207/80‑1 (MQ=255)                                                   tttCAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGt                           >  1:519248/1‑80 (MQ=255)                                                      cAGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGtcat                        >  1:519256/1‑80 (MQ=255)                                                       aGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGtcatca                      <  2:519208/81‑1 (MQ=255)                                                       aGACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGtcatc                       >  2:519254/1‑80 (MQ=255)                                                        gACAGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCaa                     <  1:519209/81‑1 (MQ=255)                                                           aGGGTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAgg                   >  2:519249/1‑80 (MQ=255)                                                            gggTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGc                  >  2:519245/1‑80 (MQ=255)                                                            gggTTTCCCATGTCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCa                 <  2:519210/81‑1 (MQ=255)                                                                        tCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCa     <  1:519212/81‑1 (MQ=255)                                                                        tCCACAACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCa     <  2:519211/81‑1 (MQ=255)                                                                          cacaACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCAtt   <  1:519213/81‑1 (MQ=255)                                                                          cacaACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCAt    <  1:519214/80‑1 (MQ=255)                                                                          cacaACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCAt    <  1:519215/80‑1 (MQ=255)                                                                           acaACTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCATTc  <  1:519216/81‑1 (MQ=255)                                                                               |                                                                             AACGTACCCATACATCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCATTC  >  NC_000913/2865033‑2865187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑