Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,883,895	G→A	Q82* (CAA→TAA)	casA ←	type I‑E CRISPR system Cascade subunit CasA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,883,895	0	G	A	100.0%	136.3 / NA	38	Q82* (CAA→TAA)	casA	type I‑E CRISPR system Cascade subunit CasA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/18);  total (20/18)

TTTGACACCTTTGGTCTGCATAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTGTTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCTTTTGCCGGGGCGATA  >  NC_000913/2883818‑2883968                                                                              |                                                                          tttGACACCTTTGGTCTGCATAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAgttatt                                                                         >  2:523220/1‑80 (MQ=255)   tGACACCTTTGGTCTGCATAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGa                                                                       <  1:523186/80‑1 (MQ=255)    gACACCTTTGGTCTGCATAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGaa                                                                      <  2:523187/80‑1 (MQ=255)    gACACCTTTGGTCTGCAT‑AAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAAc                                                                    >  1:523231/1‑81 (MQ=255)      cacCTTTGGTCTGCATAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACt                                                                   >  2:523222/1‑81 (MQ=255)           ttGGTCTGCATAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCt                                                              >  2:523240/1‑81 (MQ=255)              gTCTGCATAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTc                                                            <  2:523188/80‑1 (MQ=255)                  gCATAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGt                                                         >  1:523235/1‑79 (MQ=255)                   cATAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGt                                                         <  1:523189/78‑1 (MQ=255)                    aTAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGc                                                     <  2:523190/81‑1 (MQ=255)                       aaGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGa                                                  >  1:523228/1‑81 (MQ=255)                         gggATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGAtt                                                >  2:523237/1‑81 (MQ=255)                          ggATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTc                                               <  2:523191/81‑1 (MQ=255)                                 cTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGc                                        >  1:523233/1‑81 (MQ=255)                                    cGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGATATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGAt                                     <  1:523192/81‑1 (MQ=255)                                        aTTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTc                                  >  1:523221/1‑80 (MQ=255)                                         ttAAGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGa                                <  1:523193/81‑1 (MQ=255)                                           aaGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGaaa                              >  1:523226/1‑81 (MQ=255)                                           aaGGTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGaaa                              >  1:523232/1‑81 (MQ=255)                                             ggTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAAtt                            <  1:523195/81‑1 (MQ=255)                                             ggTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAAtt                            <  2:523194/81‑1 (MQ=255)                                              gTAGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTc                           <  1:523196/81‑1 (MQ=255)                                                aGAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCaa                         >  2:523248/1‑81 (MQ=255)                                                 gAACATATCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAAc                        >  1:523242/1‑81 (MQ=255)                                                     atatCTATCCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCa                    >  2:523229/1‑81 (MQ=255)                                                      tatctatcCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCAt                   >  1:523245/1‑81 (MQ=255)                                                       atctatcCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATAATGCGATGTCGAAATTCAACGTCATc                  >  2:523244/1‑81 (MQ=255)                                                        tctatcCACGTCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATc                  <  1:523197/80‑1 (MQ=255)                                                         ctatcCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCtt                >  2:523250/1‑81 (MQ=255)                                                           atcCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCtttt              >  1:523238/1‑81 (MQ=255)                                                            tcCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCtt                <  2:523198/78‑1 (MQ=255)                                                            tcCACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCt                 <  1:523199/77‑1 (MQ=255)                                                             ccACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCTTTTGc            >  1:523236/1‑81 (MQ=255)                                                              cACGGCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCTTTTGcc           <  2:523200/81‑1 (MQ=255)                                                                 ggCGCGATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCTTTTGCCggg        >  2:523225/1‑81 (MQ=255)                                                                    gcgATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCTTTTGCCGGGGCg     <  2:523201/81‑1 (MQ=255)                                                                      gATGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCTTTTGCCGGGGCGAt   <  2:523202/81‑1 (MQ=255)                                                                       aTGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCTTTTGCCGGGGCGAt   >  1:523256/1‑80 (MQ=255)                                                                       aTGAGTTATTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCTTTTGCCGGGGCGATa  <  2:523203/81‑1 (MQ=255)                                                                              |                                                                          TTTGACACCTTTGGTCTGCATAAAGGGATGTTCTGCGTGATTAAGGTAGAACATATCTATCCACGGCGCGATGAGTTGTTGAAACTCATCTTCAGTGAGCGGATTCATTATGCGATGTCGAAATTCAACGTCATCTTTTGCCGGGGCGATA  >  NC_000913/2883818‑2883968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑