Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	35,715	G→A	L150L (CTG→TTG)	caiD ←	crotonobetainyl‑CoA hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	35,715	0	G	A	100.0%	114.5 / NA	32	L150L (CTG→TTG)	caiD	crotonobetainyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/17);  total (15/17)

CTATCCCCCAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAGGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGATGCCCAGTTTGGCTTCCGGCAGGGCGAAGCTGGCGT  >  NC_000913/35637‑35792                                                                               |                                                                              cTATCCCCCAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAAGa                                                                             <  1:8541/81‑1 (MQ=255)   aTCCCCCAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAAGATc                                                                           >  1:8582/1‑81 (MQ=255)    tCCCCCAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAAGATc                                                                           <  2:8543/80‑1 (MQ=255)    tCCCCCAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAAGATCt                                                                          <  2:8542/81‑1 (MQ=255)      ccccAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAAGATCTTc                                                                        <  2:8544/81‑1 (MQ=255)          aaCGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAAGATCTTCgg                                                                      >  1:8611/1‑79 (MQ=255)            cGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAAGATCTTCGGCAGa                                                                  <  1:8545/81‑1 (MQ=255)             gCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAAGATCTTCGGCAGAc                                                                 >  2:8604/1‑81 (MQ=255)                        tctGCGCCCATTCGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAAGATCTTCGGCAGACGCAGCACAccg                                                      >  2:8601/1‑81 (MQ=255)                         ctGCGCCCATTCGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAAGATCTTCGGCAGACGCAGCACAccgc                                                     >  1:8612/1‑81 (MQ=255)                           gcgcCCATTCGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAAGATCTTCGGCAGACGCAGCACAccgccg                                                   >  1:8599/1‑81 (MQ=255)                            cgcCCATTCGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAAGATCTTCGGCAGACGCAGCACAccgcc                                                    <  1:8547/79‑1 (MQ=255)                            cgcCCATTCGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAAGATCTTCGGCAGACGCAGCACAccgcc                                                    <  2:8546/79‑1 (MQ=255)                                    cGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAAGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAgg                                           <  1:8548/80‑1 (MQ=255)                                     gTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAAGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGaa                                         >  1:8602/1‑81 (MQ=255)                                         gCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAAGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGAt                                     >  2:8619/1‑81 (MQ=255)                                         gCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAAGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGAt                                     >  2:8607/1‑81 (MQ=255)                                           cGGTCATCACCATTTCATTGACGATGGCAGGCGGCAAGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGATGc                                   >  1:8584/1‑81 (MQ=255)                                             gTCATCACCATTTCATTGACGATGGCAGGCGGCAAGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGATGccc                                 <  2:8549/81‑1 (MQ=255)                                               catcaCCATTTCATTGACGATGGCAGGCGGCAAGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGATGCCCAg                               >  2:8606/1‑81 (MQ=255)                                                 tcaCCATTTCATTGACGATGGCAGGCGGCAAGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGATGCCCAGtt                             <  1:8550/81‑1 (MQ=255)                                                 tcaCCATTTCATTGACGATGGCAGGCGGCAAGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGATGCCCAGtt                             <  2:8551/81‑1 (MQ=255)                                                      aTTTCATTGACGATGGCAGGCGGCAAGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGATGCCCAGTTTGGCt                        >  2:8605/1‑81 (MQ=255)                                                           aTTGACGATGGCAGGCGGCAAGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGATGCCCAGTTTGGCTTCCg                    <  1:8552/80‑1 (MQ=255)                                                               aCGATGGCAGGCGGCAAGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGATGCCCAGTTTGGCTTCCGGCAgg               <  1:8553/81‑1 (MQ=255)                                                               aCGATGGCAGGCGGCAAGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGATGCCCAGTTTGGCTTCCGGCAg                <  1:8554/80‑1 (MQ=255)                                                                cGATGGCAGGCGGCAAGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGATGCCCAGTTTGGCTTCCGGCAggg              <  1:8555/81‑1 (MQ=255)                                                                 gATGGCAGGCGGCAAGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGATGCCCAGTTTGGCTTCCGGCAGGGc             <  2:8556/81‑1 (MQ=255)                                                                     gCAGGCGGCAAGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGATGCCCAGTTTGGCTTCCGGCAGGGCGAAg         >  1:8610/1‑81 (MQ=255)                                                                          cggcAAGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGATGCCCAGTTTGGCTTCCGGCAGGGCGAAGCTGGc    >  1:8609/1‑81 (MQ=255)                                                                           ggcAAGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGATGCCCAGTTTGGCTTCCGGCAGGGCGAAGCTGGCg   >  2:8613/1‑81 (MQ=255)                                                                            gcAAGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGATGCCCAGTTTGGCTTCCGGCAGGGCGAAGCTGGCGt  <  2:8557/81‑1 (MQ=255)                                                                               |                                                                              CTATCCCCCAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCATCACCATTTCATTGACGATGGCAGGCGGCAGGATCTTCGGCAGACGCAGCACACCGCCGCTGTCAGGAACGATGCCCAGTTTGGCTTCCGGCAGGGCGAAGCTGGCGT  >  NC_000913/35637‑35792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑