Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,891,810	G→A	P30L (CCC→CTC)	cysJ ←	sulfite reductase, flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,891,810	0	G	A	100.0%	111.3 / NA	31	P30L (CCC→CTC)	cysJ	sulfite reductase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/21);  total (10/21)

CTGGCGTCGCTGCAAGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGGGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAACGGAAGCAACGCGGAAGGTGGGA  >  NC_000913/2891733‑2891885                                                                              |                                                                            ctggcgTCGCTGCAAGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGagag                                                                          >  2:524840/1‑81 (MQ=255)    gcgTCGCTGCAAGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGtt                                                                        <  2:524802/80‑1 (MQ=255)    gcgTCGCTGCAAGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGt                                                                         <  1:524801/79‑1 (MQ=255)    gcgTCGCTGCAAGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTa                                                                       <  1:524803/81‑1 (MQ=255)     cgTCGCTGCAAGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTaa                                                                      <  1:524804/81‑1 (MQ=255)      gTCGCTGCAAGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTaaa                                                                     <  1:524805/81‑1 (MQ=255)      gTCGCTGCAAGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTaaa                                                                     >  1:524852/1‑81 (MQ=255)              aaGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGt                                                             <  1:524807/81‑1 (MQ=255)              aaGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGt                                                             <  2:524806/81‑1 (MQ=255)                  gcagcaGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGgcc                                                         <  1:524808/81‑1 (MQ=255)                   cagcagGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGgccg                                                        <  1:524809/81‑1 (MQ=255)                   cagcagGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGgccg                                                        <  2:524810/81‑1 (MQ=255)                      cagGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCt                                                     <  1:524812/81‑1 (MQ=255)                      cagGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCt                                                     <  2:524811/81‑1 (MQ=255)                       agGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCt                                                     >  1:524847/1‑80 (MQ=255)                         gctgctGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTg                                                    >  1:524841/1‑79 (MQ=255)                              tgATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGt                                             >  1:524860/1‑81 (MQ=255)                                aTTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGt                                             <  1:524813/79‑1 (MQ=255)                                   gAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAg                                        >  2:524849/1‑81 (MQ=255)                                       aCGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGc                                    >  1:524851/1‑81 (MQ=255)                                            ccAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCgg                               >  1:524846/1‑81 (MQ=255)                                               gAAATAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGtt                            <  1:524814/81‑1 (MQ=255)                                                aaaTAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGtt                            >  2:524850/1‑80 (MQ=255)                                                 aaTAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCa                          <  2:524815/81‑1 (MQ=255)                                                   tAGCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAAc                        <  2:524816/81‑1 (MQ=255)                                                     gCCAGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAACgg                      <  1:524817/81‑1 (MQ=255)                                                      ccAGAAACCCAGGCAAGCTGGGTGAGAGTTAGATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAACGGa                     <  2:524818/81‑1 (MQ=255)                                                        aGAAACCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAACGGAAg                   >  1:524853/1‑81 (MQ=255)                                                          aaaCCCAGGCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAACGGAAGc                  >  2:524855/1‑80 (MQ=255)                                                                 ggCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAACGGAAGCAACGCGGa          <  1:524820/81‑1 (MQ=255)                                                                 ggCAAGCTGGGTGAGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAACGGAAGCAACGCGGa          <  1:524819/81‑1 (MQ=255)                                                                 ggCAAGCTGGGTGAGAGTAAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAACGGAAGCAACGCGGa          <  2:524821/81‑1 (MQ=255)                                                                         gggtgagAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAACGGAAGCAACGCGGAAGGTGGGa  >  1:524854/1‑81 (MQ=255)                                                                              |                                                                            CTGGCGTCGCTGCAAGCGCAGCAGGCTGCTGATTGAGTACGCCCCAGAAATAGCCAGAAACCCAGGCAAGCTGGGTGGGAGTTAAATCGGTCGTGGCCGCCTGAAGGCGTGCCAGTTGCTCCGGGTTCAACGGAAGCAACGCGGAAGGTGGGA  >  NC_000913/2891733‑2891885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑