Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,928,914	C→T	S229F (TCT→TTT)	sdaC →	serine:H(+) symporter SdaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,928,914	0	C	T	100.0%	202.4 / NA	56	S229F (TCT→TTT)	sdaC	serine:H(+) symporter SdaC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/32);  total (24/32)

CGGAAACGGTCTGTGGATGACCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTCTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGAAGATCCTGGCATTCGCA  >  NC_000913/2928837‑2928990                                                                              |                                                                             cGGAAACGGTCTGTGGATGACCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCttttc                                                                           <  1:532526/81‑1 (MQ=255)    aaaCGGTCTGTGGATGACCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCAtcttttcttt                                                                        >  2:532586/1‑81 (MQ=255)    aaaCGGTCTGTGGATGACCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCAtcttttcttt                                                                        >  1:532587/1‑81 (MQ=255)     aaCGGTCTGTGGATGACCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTtttctttc                                                                       >  1:532580/1‑81 (MQ=255)       cGGTCTGTGGATGACCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGc                                                                     <  2:532527/81‑1 (MQ=255)       cGGTCTGTGGATGACCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGc                                                                     >  1:532572/1‑81 (MQ=255)        ggTCTGTGGATGACCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGcc                                                                    >  1:532576/1‑81 (MQ=255)         gTCTGTGGATGACCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCg                                                                   <  1:532528/81‑1 (MQ=255)         gTCTGTGGATGACCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCg                                                                   <  1:532529/81‑1 (MQ=255)           cTGTGGATGACCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGtt                                                                 >  2:532598/1‑81 (MQ=255)              tgGATGACCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGtt                                                                 <  1:532530/78‑1 (MQ=255)                 aTGACCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAg                                                           <  2:532531/81‑1 (MQ=255)                 aTGACCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAg                                                           >  2:532584/1‑81 (MQ=255)                  tGACCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGc                                                          <  2:532532/81‑1 (MQ=255)                    aCCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCg                                                         <  1:532533/80‑1 (MQ=255)                       cTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGaa                                                     <  1:532534/81‑1 (MQ=255)                          tggctggcAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAag                                                    <  2:532535/79‑1 (MQ=255)                           ggctggcAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAagag                                                  >  2:532585/1‑80 (MQ=255)                            gctggcAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGt                                                 <  1:532536/80‑1 (MQ=255)                            gctggcAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTa                                                >  1:532581/1‑81 (MQ=255)                                gcAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACgg                                             <  1:532537/80‑1 (MQ=255)                                gcAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGc                                            >  1:532611/1‑81 (MQ=255)                                gcAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGc                                            <  2:532538/81‑1 (MQ=255)                                 cAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGc                                            >  2:532605/1‑80 (MQ=255)                                 cAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCg                                           <  2:532539/81‑1 (MQ=255)                                 cAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTCTCGCCGTTGCGAAGCGTGAAGAGTACGGCg                                           <  2:532540/81‑1 (MQ=255)                                  aaTTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTCTCGCCGTTGCGAAGCGTGAAGAGTACGGCGa                                          >  2:532589/1‑81 (MQ=255)                                   aTTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGat                                         <  1:532541/81‑1 (MQ=255)                                   aTTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGa                                          >  2:532575/1‑80 (MQ=255)                                    ttCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGata                                        <  1:532542/81‑1 (MQ=255)                                      ccGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGatat                                       <  2:532543/80‑1 (MQ=255)                                       cGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATg                                      <  2:532545/80‑1 (MQ=255)                                       cGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATg                                      <  2:532544/80‑1 (MQ=255)                                        ggTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATgg                                     >  2:532601/1‑80 (MQ=255)                                        ggTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGc                                    <  1:532546/81‑1 (MQ=255)                                            aTGGTGTTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGcaga                                 <  1:532548/80‑1 (MQ=255)                                            aTGGTGTTCTCGATCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGcaga                                 <  1:532547/80‑1 (MQ=255)                                               gtgtTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGcagaacag                             <  2:532549/81‑1 (MQ=255)                                                tgtTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGcagaacaga                            <  1:532550/81‑1 (MQ=255)                                                tgtTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGcagaacag                             >  2:532602/1‑80 (MQ=255)                                                 gtTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGcagaacaga                            >  1:532604/1‑80 (MQ=255)                                                 gtTCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGcagaacaga                            >  2:532615/1‑80 (MQ=255)                                                  ttCTCGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGaaa                          >  2:532609/1‑81 (MQ=255)                                                    ctcGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATg                        >  1:532590/1‑81 (MQ=255)                                                      cGTTCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCt                      >  1:532595/1‑81 (MQ=255)                                                        ttCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCg                    <  2:532552/81‑1 (MQ=255)                                                        ttCAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCg                    <  1:532551/81‑1 (MQ=255)                                                          cAACCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGa                   <  2:532553/80‑1 (MQ=255)                                                           aaCCACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCg                    >  2:532613/1‑78 (MQ=255)                                                             ccACTCTCCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGAAGAt               <  1:532554/81‑1 (MQ=255)                                                                 tctcCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGAAGATCCTg           >  1:532600/1‑81 (MQ=255)                                                                  ctcCGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGTGTACGGCGATATGGCAGAACAGAATTGCTCGAAGACCCTgg          >  1:532617/1‑81 (MQ=255)                                                                   tcCGATCATCTTTTCTTTCCCCGTTCCTAAGCGTGAAGAGTACGTCCCTATTGCATAACAGTAATTCTCCAAGATCCTGGc         >  2:977421/1‑81 (MQ=255)                                                                     cGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGAAGATCCTGGc         <  2:532556/79‑1 (MQ=255)                                                                     cGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGAAGATCCTGGc         <  2:532555/79‑1 (MQ=255)                                                                     cGATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGAAGATCCTGGCAt       >  1:532597/1‑81 (MQ=255)                                                                      gATCATCTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGAAGATCCTGGCAtt      >  1:532594/1‑81 (MQ=255)                                                                       atcatcTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGAAGATCCTGGCATTc     <  1:532557/81‑1 (MQ=255)                                                                        tcatcTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGAAGATCCTGGCATTCg    >  2:532630/1‑81 (MQ=255)                                                                          atcTTTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGAAGATCCTGGCATTCGca  <  1:532558/81‑1 (MQ=255)                                                                              |                                                                             CGGAAACGGTCTGTGGATGACCCTGTGGCTGGCAATTCCGGTAATGGTGTTCTCGTTCAACCACTCTCCGATCATCTCTTCTTTCGCCGTTGCGAAGCGTGAAGAGTACGGCGATATGGCAGAACAGAAATGCTCGAAGATCCTGGCATTCGCA  >  NC_000913/2928837‑2928990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑