Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,945,771	C→T	A24A (GCG→GCA)	tcdA ←	tRNA threonylcarbamoyladenosine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,945,771	0	C	T	100.0%	82.8 / NA	24	A24A (GCG→GCA)	tcdA	tRNA threonylcarbamoyladenosine dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/13);  total (11/13)

CGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGACATAGCA  >  NC_000913/2945693‑2945846                                                                               |                                                                            cgcCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGc                                                                           <  1:536252/81‑1 (MQ=255)         cTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCAc                                                                   >  2:536297/1‑81 (MQ=255)            ccgccgCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCa                                                                 >  2:536302/1‑80 (MQ=255)            ccgccgCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCa                                                                 <  2:536253/80‑1 (MQ=255)              gccgccCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATa                                                               >  2:536286/1‑80 (MQ=255)              gccgccCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATAc                                                              <  2:536254/81‑1 (MQ=255)               ccgccCAGGAACCGACACCACCGATACCAACCACCCAAATATGCGCGTCAGCAAACAGATGCAATGCTTTTTCACCATa                                                               >  1:536285/1‑79 (MQ=255)                  cccAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGAc                                                          <  1:536255/81‑1 (MQ=255)                     aGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGAcgcg                                                       <  2:536256/81‑1 (MQ=255)                        aaCCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCtg                                                    >  2:536257/1‑81 (MQ=255)                           cGACGCCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGc                                                 <  1:536258/81‑1 (MQ=255)                                 caccGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCaa                                           <  1:536259/81‑1 (MQ=255)                                    cGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAAc                                        <  1:536260/81‑1 (MQ=255)                                     gATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACg                                       >  1:536295/1‑81 (MQ=255)                                       tACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCt                                     <  1:536261/81‑1 (MQ=255)                                        accaaccaCACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCt                                     >  1:536291/1‑80 (MQ=255)                                          caaccaCACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGAc                                  <  2:536262/81‑1 (MQ=255)                                          caaccaCACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGAc                                  >  2:536292/1‑81 (MQ=255)                                             ccaCACAAATATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGcc                               <  2:536263/81‑1 (MQ=255)                                                    aaTATGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCAt                         >  2:536296/1‑80 (MQ=255)                                                        tGCGCGTCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACt                     <  1:536264/80‑1 (MQ=255)                                                              tCAGCAAACAGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTAcc              <  1:536265/81‑1 (MQ=255)                                                                       aGTTGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGACa       >  2:536312/1‑79 (MQ=255)                                                                          tGCAATGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGACATAGCa  >  1:536314/1‑81 (MQ=255)                                                                               |                                                                            CGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCTTTTTCACCATACAGACGCGCTGTGCCACCAAAACGCTGACGCCATGCATCACTAATTACCACAGACATAGCA  >  NC_000913/2945693‑2945846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑