Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,948,577	G→C	A145G (GCC→GGC)	amiC ←	N‑acetylmuramoyl‑L‑alanine amidase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,948,577	0	G	C	100.0%	119.5 / NA	35	A145G (GCC→GGC)	amiC	N‑acetylmuramoyl‑L‑alanine amidase C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (13/22);  total (13/22)

GGCACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGGCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGTTTTACGTTT  >  NC_000913/2948499‑2948654                                                                               |                                                                              ggcACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGccc                                                                             >  2:536832/1‑81 (MQ=255)  gcACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCg                                                                            >  2:536838/1‑81 (MQ=255)  gcACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCg                                                                            <  2:536793/81‑1 (MQ=255)  gcACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCg                                                                            <  2:536792/81‑1 (MQ=255)        gCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATa                                                                        >  1:536844/1‑79 (MQ=255)         cTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATaga                                                                      <  2:536794/80‑1 (MQ=255)                  gTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCAc                                                            <  1:536795/81‑1 (MQ=255)                   tCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCAcc                                                           <  2:536797/81‑1 (MQ=255)                   tCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCAcc                                                           <  2:536796/81‑1 (MQ=255)                   tCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCAc                                                            >  2:536848/1‑80 (MQ=255)                         ttgttgTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGc                                                     >  1:536839/1‑81 (MQ=255)                          tgttgtAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGc                                                     <  1:536798/80‑1 (MQ=255)                                       cagcagcgccagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGc                                       >  2:536846/1‑81 (MQ=255)                                        agcagcgccagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGc                                       >  2:536842/1‑80 (MQ=255)                                          cagcgccagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGAc                                    <  2:536799/81‑1 (MQ=255)                                             cgccagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCgg                                 <  2:536800/81‑1 (MQ=255)                                              gccagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGgc                                >  1:536840/1‑81 (MQ=255)                                              gccagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGgc                                <  1:536801/81‑1 (MQ=255)                                                cagcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGgcgc                              <  1:536802/81‑1 (MQ=255)                                                 agcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGgcgc                              >  2:536841/1‑80 (MQ=255)                                                 agcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCa                             <  2:536803/81‑1 (MQ=255)                                                  gcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGgcgc                              >  2:536852/1‑79 (MQ=255)                                                  gcagcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCaa                            <  1:536804/81‑1 (MQ=255)                                                    agcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAgg                          >  1:536843/1‑81 (MQ=255)                                                    agcgGGTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAgg                          >  2:536849/1‑81 (MQ=255)                                                       gggTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCa                       <  1:536806/81‑1 (MQ=255)                                                       gggTCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCa                       <  1:536805/81‑1 (MQ=255)                                                          tCCTGCATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAAc                    <  2:536807/81‑1 (MQ=255)                                                               cATATCCTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCt                <  1:536808/80‑1 (MQ=255)                                                                     cTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGtttt         >  2:536866/1‑81 (MQ=255)                                                                     cTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGttt         <  1:536810/81‑1 (MQ=255)                                                                     cTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGttt         <  1:536811/81‑1 (MQ=255)                                                                     cTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGtt          <  2:536809/80‑1 (MQ=255)                                                                     cTGTGCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGtt          <  1:536812/80‑1 (MQ=255)                                                                       gtgCATTGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGTTTTa       >  2:536856/1‑81 (MQ=255)                                                                            ttGCCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGTTTTACGttt  <  1:536813/81‑1 (MQ=255)                                                                               |                                                                              GGCACCTGCTTTTCGAGGTCGCCTTTGTTGTAATCCTCCAGCAGCGCCAGCAGCGGGTCCTGCATATCCTGTGCATTGGCCGGATAGAGGTCCATCACCAGACGCTCTTTAAACCCGGCGACCGGCGCAAGGGCAAACAGCTGCGGTTTTACGTTT  >  NC_000913/2948499‑2948654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑