Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,989,382	A→G	T27A (ACT→GCT)	yqeJ →	protein YqeJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,989,382	0	A	G	100.0%	36.3 / NA	12	T27A (ACT→GCT)	yqeJ	protein YqeJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/6);  total (6/6)

TGATTGATTATAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATC  >  NC_000913/2989305‑2989457                                                                              |                                                                            tgattgatTATAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACGCTg                                                                          >  1:546022/1‑81 (MQ=255)             aaaaaaaCTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTATTACGCTGCAGAAAAAATg                                                               <  1:546004/80‑1 (MQ=255)              aaaaaaCTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACGCTGCAGAAAAAATGa                                                              >  1:546024/1‑80 (MQ=255)                   aCTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACGCTGCAGAAAAAATGatatat                                                         >  1:546021/1‑80 (MQ=255)                    cTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACGCTGCAGAAAAAATGATATATaa                                                       <  2:546005/81‑1 (MQ=255)                              atttTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACGCTGCAGAAAAAATGATATATAACGAAACATg                                              >  1:546020/1‑80 (MQ=255)                              atttTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACGCTGCAGAAAAAATGATATATAACGAAACATg                                              >  1:546030/1‑80 (MQ=255)                                         tATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACGCTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAAtt                                   <  1:546006/80‑1 (MQ=255)                                                            ttCACTGTCTATTCTTACGCTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGAc                  <  1:546007/78‑1 (MQ=255)                                                            ttCACTGTCTATTCTTACGCTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGAc                  <  2:546008/78‑1 (MQ=255)                                                              cACTGTCTATTCTTACGCTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAg               <  2:546009/79‑1 (MQ=255)                                                                         cTTACGCTGCAGAAAAAATGATATATAACGAAACATGCACTACAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATc  >  1:546026/1‑81 (MQ=255)                                                                              |                                                                            TGATTGATTATAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACTGCAGAAAAAATGATATATAACGAAACATGCACTGCAAATTGGGTAATTTTTAATGACCAGGGACGAGCAAATC  >  NC_000913/2989305‑2989457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑