Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,007,291	G→T	E344* (GAA→TAA)	ygeW →	putative carbamoyltransferase YgeW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,007,291	0	G	T	100.0%	155.5 / NA	43	E344* (GAA→TAA)	ygeW	putative carbamoyltransferase YgeW
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/29);  total (14/29)

GAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCC  >  NC_000913/3007213‑3007368                                                                               |                                                                              gAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTaa                                                                             <  2:548968/81‑1 (MQ=255)   aCTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGt                                                                           <  1:548970/81‑1 (MQ=255)   aCTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGt                                                                           <  2:548969/81‑1 (MQ=255)     tGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGa                                                                         >  1:549032/1‑81 (MQ=255)        cccGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACt                                                                       >  1:549035/1‑80 (MQ=255)        cccGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTg                                                                      <  1:548971/81‑1 (MQ=255)        cccGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTg                                                                      <  2:548972/81‑1 (MQ=255)          cGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGaa                                                                    <  1:548973/81‑1 (MQ=255)           gTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAg                                                                   <  2:548974/81‑1 (MQ=255)            tGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAgg                                                                  <  2:548975/81‑1 (MQ=255)             gATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGc                                                                 >  2:549020/1‑81 (MQ=255)                ggCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTa                                                              >  2:549051/1‑81 (MQ=255)                ggCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTa                                                              >  1:549043/1‑81 (MQ=255)                   gAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTc                                                           <  1:548976/81‑1 (MQ=255)                    aaGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCg                                                          <  2:548978/81‑1 (MQ=255)                    aaGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCg                                                          <  1:548977/81‑1 (MQ=255)                     aGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGa                                                         <  2:548979/81‑1 (MQ=255)                        ccTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGaaa                                                       >  2:549048/1‑80 (MQ=255)                            tACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATa                                                   <  2:548980/80‑1 (MQ=255)                            tACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATAc                                                  <  1:548981/81‑1 (MQ=255)                              cATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATAcc                                                 <  2:548983/80‑1 (MQ=255)                              cATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCg                                                <  1:548982/81‑1 (MQ=255)                                    ctgcctgcCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGc                                          <  1:548984/81‑1 (MQ=255)                                      gcctgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGcta                                        >  1:549049/1‑81 (MQ=255)                                         tgccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGctacc                                      >  1:549021/1‑80 (MQ=255)                                             aGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACa                                  >  2:549039/1‑80 (MQ=255)                                              gCTGATATCAGCGGCGTAACCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACaaa                                <  2:548985/81‑1 (MQ=255)                                                  atatCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAaagaa                             <  2:548986/80‑1 (MQ=255)                                                    atCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGc                           >  1:549040/1‑80 (MQ=255)                                                    atCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGc                           >  2:549036/1‑80 (MQ=255)                                                     tCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGcc                          <  2:548988/80‑1 (MQ=255)                                                     tCAGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGcc                          <  1:548987/80‑1 (MQ=255)                                                       aGCGGCGTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAg                        <  2:548989/80‑1 (MQ=255)                                                         cggcgTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTg                     <  2:548990/81‑1 (MQ=255)                                                            cgTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGaa                  <  2:548991/81‑1 (MQ=255)                                                            cgTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGaa                  <  2:548992/81‑1 (MQ=255)                                                             gTATCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAg                 >  1:549028/1‑81 (MQ=255)                                                                tCCTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGcc               <  2:548993/80‑1 (MQ=255)                                                                  cTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCtt             <  1:548994/80‑1 (MQ=255)                                                                  cTGTAAAGAAGGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTta            >  1:549046/1‑81 (MQ=255)                                                                       aagaagGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATatca       >  2:549044/1‑81 (MQ=255)                                                                        agaagGTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATatcat      <  1:548995/81‑1 (MQ=255)                                                                            ggTTAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATcgcc  <  1:548996/81‑1 (MQ=255)                                                                               |                                                                              GAACTGACCCGTGATGGCGAAGCCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCC  >  NC_000913/3007213‑3007368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑