Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,020,030	C→T	N290N (AAC→AAT)	ssnA →	putative aminohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,020,030	0	C	T	100.0%	153.9 / NA	42	N290N (AAC→AAT)	ssnA	putative aminohydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/23);  total (19/23)

GGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTC  >  NC_000913/3019954‑3020107                                                                             |                                                                              ggCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAATATg                                                                            <  2:551794/80‑1 (MQ=255) ggCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAATATg                                                                            >  2:551836/1‑80 (MQ=255) ggCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAATATGa                                                                           <  1:551795/81‑1 (MQ=255)   cTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAATATGaac                                                                         <  1:551796/81‑1 (MQ=255)   cTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAATATGaac                                                                         <  2:551797/81‑1 (MQ=255)           gTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAATATGAACAACCAt                                                                   <  2:551798/79‑1 (MQ=255)           gTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAATATGAACAACCAt                                                                   <  1:551799/79‑1 (MQ=255)                  gATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTa                                                           >  2:551860/1‑80 (MQ=255)                     gACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAAc                                                       >  1:551857/1‑81 (MQ=255)                      aCATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAAc                                                       >  2:551848/1‑80 (MQ=255)                        aTCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCAt                                                    <  1:551800/81‑1 (MQ=255)                              cTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCtt                                              <  1:551802/81‑1 (MQ=255)                              cTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCtt                                              <  1:551801/81‑1 (MQ=255)                              cTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCtt                                              <  2:551803/81‑1 (MQ=255)                                aCTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAg                                            >  1:551863/1‑81 (MQ=255)                                 cTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTa                                             <  2:551806/79‑1 (MQ=255)                                 cTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGc                                           <  1:551804/81‑1 (MQ=255)                                 cTCAAACAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTa                                             <  1:551805/79‑1 (MQ=255)                                   cAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGa                                         >  2:551866/1‑81 (MQ=255)                                        aGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATcc                                    >  1:551856/1‑81 (MQ=255)                                          cgcgATGCGTTCCTGGTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCg                                   >  2:551908/1‑80 (MQ=255)                                                 cGTTCCTGGTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTg                            <  2:551807/80‑1 (MQ=255)                                                  gTTCCTGGTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGc                          >  1:551854/1‑81 (MQ=255)                                                   ttCCTGGTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCg                         <  2:551808/81‑1 (MQ=255)                                                     ccTGGTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGt                        <  2:551809/80‑1 (MQ=255)                                                      cTGGTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTg                      >  1:551851/1‑81 (MQ=255)                                                       tGGTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTgg                     >  1:551864/1‑81 (MQ=255)                                                         gTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTggg                    >  1:551868/1‑80 (MQ=255)                                                         gTGCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGa                   <  1:551810/81‑1 (MQ=255)                                                           gCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAac                 >  1:551865/1‑81 (MQ=255)                                                           gCATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAac                 >  1:551869/1‑81 (MQ=255)                                                            cATAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAacg                <  1:551811/81‑1 (MQ=255)                                                             aTAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAacg                >  2:551853/1‑80 (MQ=255)                                                              tAACGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAacgga              >  1:551872/1‑81 (MQ=255)                                                                aCGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAacggac             <  1:551813/80‑1 (MQ=255)                                                                aCGCCCGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAacggac             <  1:551812/80‑1 (MQ=255)                                                                   cccGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCa         <  2:551815/81‑1 (MQ=255)                                                                   cccGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCa         <  1:551814/81‑1 (MQ=255)                                                                     cGTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCAtt       >  1:551871/1‑81 (MQ=255)                                                                      gTTCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTg      >  2:551878/1‑81 (MQ=255)                                                                       ttCAAATATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTgg     >  1:551876/1‑81 (MQ=255)                                                                          aaaTATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTc  <  2:551816/81‑1 (MQ=255)                                                                             |                                                                              GGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATCTGGCGTTGGGAACGGACGGCATTGGTTC  >  NC_000913/3019954‑3020107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑