Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,028,256	T→C	M379T (ATG→ACG)	ghxQ →	guanine/hypoxanthine transporter GhxQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,028,256	0	T	C	100.0%	102.1 / NA	30	M379T (ATG→ACG)	ghxQ	guanine/hypoxanthine transporter GhxQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/11);  total (19/11)

TTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGCTCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGCTATGGCTGGCCTGGTGTGTGCCGTGTTCATC  >  NC_000913/3028178‑3028332                                                                               |                                                                             ttCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGACGt                                                                            >  1:553815/1‑81 (MQ=255)   cTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGACGtt                                                                           >  2:553817/1‑80 (MQ=255)      tttttATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGACGTTAAg                                                                        >  1:553809/1‑80 (MQ=255)         ttATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGACGTTAAGTAAc                                                                    >  2:553827/1‑81 (MQ=255)                cGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGa                                                             <  1:553777/81‑1 (MQ=255)                  cTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAg                                                           <  2:553778/81‑1 (MQ=255)                    gTCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAGCt                                                         >  2:553842/1‑81 (MQ=255)                     tCATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAGCTg                                                        <  2:553779/81‑1 (MQ=255)                      cATTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAGCTg                                                        >  2:553818/1‑80 (MQ=255)                       aTTTTTGATCCCTGGTTACGCCACTGCACCCGATCTGACGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAGCTGGa                                                      >  2:553816/1‑81 (MQ=255)                        tttttGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAGCTGGAt                                                     >  2:553830/1‑81 (MQ=255)                           ttGATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAGCTGGAttt                                                   >  1:553820/1‑80 (MQ=255)                             gATCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAGCTGGATTTCaa                                                >  2:553804/1‑81 (MQ=255)                              aTCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAGCTGGATTTCAAt                                               >  2:553843/1‑81 (MQ=255)                              aTCCCTGGTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAGCTGGATTTCAAt                                               >  1:553835/1‑81 (MQ=255)                                    ggTTACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAGCTGGATTTCAATGAttt                                          >  1:553839/1‑80 (MQ=255)                                      ttACGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTAt                                       <  1:553780/81‑1 (MQ=255)                                        aCGCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTAtt                                      <  1:553781/80‑1 (MQ=255)                                          gCCACTGCACCCGATCTGATGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGAc                                   <  2:553782/81‑1 (MQ=255)                                             aCTGCACCCGATCTGATGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGCt                                <  1:553783/81‑1 (MQ=255)                                                 cACCCGATCTGATGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGCTAtg                             <  2:553784/80‑1 (MQ=255)                                                  aCCCGATCTGATGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGCTAtggc                           >  1:553826/1‑81 (MQ=255)                                                           gATGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGCTATGGCTGGCCTGgt                  >  2:553832/1‑81 (MQ=255)                                                            aTGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGCTATGGCTGGCCTGgt                  <  2:553785/80‑1 (MQ=255)                                                             tGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGCTATGGCTGGCCTGgtgt                >  2:553837/1‑81 (MQ=255)                                                             tGTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGCTATGGCTGGCCTGgtgt                <  2:553786/81‑1 (MQ=255)                                                              gTACGTAGGTTTGCTGACGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGCTATGGCTGGCCTGgtgtg               >  1:553838/1‑81 (MQ=255)                                                                         tGCTGACGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGCTATGGCTGGCCTGGTGTGTGCCGTGTTCa    >  2:553846/1‑81 (MQ=255)                                                                         tGCTGACGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGCTATGGCTGGCCTGGTGTGTGCCGTGTTCa    >  2:553847/1‑81 (MQ=255)                                                                           cTGACGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGCTATGGCTGGCCTGGTGTGTGCCGTGTTCATc  <  2:553787/81‑1 (MQ=255)                                                                               |                                                                             TTCTGTTTTTATCACCGCTGTCATTTTTGATCCCTGGTTACGCCACTGCACCCGCTCTGATGTACGTAGGTTTGCTGATGTTAAGTAACGTCTCGAAGCTGGATTTCAATGATTTTATTGACGCTATGGCTGGCCTGGTGTGTGCCGTGTTCATC  >  NC_000913/3028178‑3028332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑