Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,030,090	A→G	V281A (GTC→GCC)	ygfT ←	fused putative oxidoreductase, Fe‑S subunit and nucleotide‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,030,090	0	A	G	100.0%	102.3 / NA	30	V281A (GTC→GCC)	ygfT	fused putative oxidoreductase, Fe‑S subunit and nucleotide‑binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/17);  total (13/17)

GCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAATTTCT  >  NC_000913/3030020‑3030167                                                                       |                                                                              gCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCg                                                                      <  1:554215/80‑1 (MQ=255)    gACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGt                                                                   >  2:554250/1‑80 (MQ=255)      cATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGAt                                                                <  1:554216/81‑1 (MQ=255)        tCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCt                                                              <  1:554217/81‑1 (MQ=255)        tCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCt                                                              <  1:554218/81‑1 (MQ=255)          gggACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCttt                                                            >  2:554260/1‑81 (MQ=255)          gggACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCttt                                                            >  1:554258/1‑81 (MQ=255)            gACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTc                                                           >  1:554251/1‑80 (MQ=255)             aCGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCaa                                                         <  1:554219/81‑1 (MQ=255)                ccAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAg                                                       >  2:554268/1‑80 (MQ=255)                  aGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTa                                                     >  1:554243/1‑80 (MQ=255)                    cccATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACAt                                                  <  1:554220/81‑1 (MQ=255)                       aTCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATgcg                                               <  1:554221/81‑1 (MQ=255)                            cAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCtt                                           >  2:554255/1‑80 (MQ=255)                            cAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTc                                          <  2:554222/81‑1 (MQ=255)                              gcgcGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTcac                                        <  2:554223/81‑1 (MQ=255)                                gcgGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTcaca                                       >  2:554253/1‑80 (MQ=255)                                  ggTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAg                                    <  2:554224/81‑1 (MQ=255)                                   gTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGa                                   <  2:554225/81‑1 (MQ=255)                                       cGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGt                               >  1:554252/1‑81 (MQ=255)                                       cGGTGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGt                               >  2:554264/1‑81 (MQ=255)                                          tGATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCt                            <  2:554226/81‑1 (MQ=255)                                           gATGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCtg                           >  2:554256/1‑81 (MQ=255)                                            aTGTAGCGTTCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCtgt                          <  2:554227/81‑1 (MQ=255)                                                    ttCCAGATTACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATAc                  <  2:554228/81‑1 (MQ=255)                                                           ttACCGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCg           <  2:554229/81‑1 (MQ=255)                                                             aCCGATAGAGGCTGCGCCCGAGTGATCTTTCAAGGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGc          <  1:554230/80‑1 (MQ=255)                                                               cGATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCaaa       <  1:554231/81‑1 (MQ=255)                                                                gATAGAGGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAAt      >  2:554266/1‑81 (MQ=255)                                                                   agagGCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAATTTc   >  2:554254/1‑81 (MQ=255)                                                                    gaggCTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAATTTCt  >  2:554257/1‑81 (MQ=255)                                                                       |                                                                              GCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTGATCTTTCAAAGTACATGCGCCTTCACAAAGACGGTCCTGTGGACATACCCTGCCGCAAATTTCT  >  NC_000913/3030020‑3030167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑