Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,031,950	T→C	I195T (ATC→ACC)	uacT →	urate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,031,950	0	T	C	100.0%	90.6 / NA	27	I195T (ATC→ACC)	uacT	urate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/16);  total (11/16)

GCCGGAGGTAAAGGGAATCCGCAATATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTT  >  NC_000913/3031877‑3032025                                                                          |                                                                            gccgGAGGTAAAGGGAATCCGCAATATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTACCTTGCt                                                                       >  1:554681/1‑80 (MQ=255)  ccgGAGGTAAAGGGAATCCGCAATATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTACCTTGCTCa                                                                     <  2:554632/81‑1 (MQ=255)   cgGAGGTAAAGGGAATCCGCAATATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTACCTTGCTCAt                                                                    >  1:554682/1‑81 (MQ=255)    ggAGGTAAAGGGAATCCGCAATATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTACCTTGCTCAtt                                                                   <  2:554633/81‑1 (MQ=255)       ggTAAAGGGAATCCGCAATATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTACCTTGCTCATTACt                                                                <  2:554634/81‑1 (MQ=255)           aaGGGAATCCGCAATATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTACCTTGCTCATTACTCGCt                                                            <  2:554635/81‑1 (MQ=255)             gggAATCCGCAATATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTACCTTGCTCATTACTCGCTAt                                                          <  1:554636/81‑1 (MQ=255)              ggAATCCGCAATATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTACCTTGCTCATTACTCGCTATg                                                         <  1:554637/81‑1 (MQ=255)                aaTCCGCAATATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTACCTTGCTCATTACTCGCTATGc                                                        >  2:554638/1‑80 (MQ=255)                     gCAATATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTACCTTGCTCATTACTCGCTATGCGAAAg                                                   >  1:554679/1‑80 (MQ=255)                        atatGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTACCTTGCTCATTACTCGCTATGCGAAAGGAtt                                               <  2:554639/81‑1 (MQ=255)                         tatGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTACCTTGCTCATTACTCGCTATGCGAAAGGAttt                                              <  1:554640/81‑1 (MQ=255)                                aTCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTACCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCa                                       <  1:554641/81‑1 (MQ=255)                                   ccGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTACCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACg                                    >  2:554693/1‑81 (MQ=255)                                      tttatttaGGTATCTCCTTTGCCGTCTTAATTTTTACCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCg                                 >  1:554678/1‑81 (MQ=255)                                         atttaGGTATCTCCTTTGCCGTCTTAATTTTTACCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCg                              >  2:554713/1‑81 (MQ=255)                                          tttaGGTATCTCCTTTGCCGTCTTAATTTTTACCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGt                             <  1:554642/81‑1 (MQ=255)                                            taGGTATCTCCTTTGCCGTCTTAATTTTTACCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTa                            <  1:554643/80‑1 (MQ=255)                                              ggTATCTCCTTTGCCGTCTTAATTTTTACCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTa                         >  2:554684/1‑81 (MQ=255)                                                      cTTTGCCGTCTTAATTTTTACCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTgg                     <  2:554644/77‑1 (MQ=255)                                                      cTTTGCCGTCTTAATTTTTACCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGa                  <  2:554645/80‑1 (MQ=255)                                                          gCCGTCTTAATTTTTACCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGt              <  2:554646/80‑1 (MQ=255)                                                          gCCGTCTTAATTTTTACCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTa             >  2:554690/1‑81 (MQ=255)                                                          gCCGTCTTAATTTTTACCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTa             >  1:554683/1‑81 (MQ=255)                                                           ccGTCTTAATTTTTACCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTAt            <  2:554647/81‑1 (MQ=255)                                                                  aaTTTTTACCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCtt     >  1:554685/1‑81 (MQ=255)                                                                      tttACCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCttttt  <  2:554648/80‑1 (MQ=255)                                                                          |                                                                            GCCGGAGGTAAAGGGAATCCGCAATATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTT  >  NC_000913/3031877‑3032025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑