Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,032,582	C→T	P406S (CCG→TCG)	uacT →	urate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,032,582	0	C	T	100.0%	120.3 / NA	34	P406S (CCG→TCG)	uacT	urate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/22);  total (12/22)

GCGCTGTAACTACACCACCAACCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTCCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTACTGCAACCGCTGCTACATAGCGGCATTATGCTCGC  >  NC_000913/3032512‑3032658                                                                       |                                                                             gcgcTGTAACTACACCACCAACCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTTCGACGctct                                                                     <  1:554766/80‑1 (MQ=255)  cgcTGTAACTACACCACCAACCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTTCGACGctctct                                                                   <  1:554767/81‑1 (MQ=255)     tGTAACTACACCACCAACCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCAc                                                                >  2:554803/1‑81 (MQ=255)       tAACTACACCACCAACCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACg                                                               <  2:554768/80‑1 (MQ=255)       tAACTACACCACCAACCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGa                                                              >  2:554834/1‑81 (MQ=255)       tAACTACACCACCAACCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGa                                                              >  2:554812/1‑81 (MQ=255)         aCTACACCACCAACCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGAt                                                             <  2:554769/80‑1 (MQ=255)         aCTACACCACCAACCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGAt                                                             <  1:554770/80‑1 (MQ=255)             caccacCAACCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGAtttct                                                         <  2:554772/80‑1 (MQ=255)             caccacCAACCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGAtttc                                                          <  2:554771/79‑1 (MQ=255)                 accaACCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGAtttcttttct                                                    <  2:554773/81‑1 (MQ=255)                   caACCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGAtttcttttct                                                    >  2:554825/1‑79 (MQ=255)                    aaCCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGATTTCTTTTCTAAg                                                 <  1:554774/81‑1 (MQ=255)                    aaCCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGATTTCTTTTCTAAg                                                 >  1:554822/1‑81 (MQ=255)                    aaCCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGATTTCTTTTCTAAg                                                 >  1:554818/1‑81 (MQ=255)                            cAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGc                                         <  1:554775/81‑1 (MQ=255)                             aaCCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGcc                                        <  2:554776/81‑1 (MQ=255)                              aCCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCg                                       <  2:554777/81‑1 (MQ=255)                               ccTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCg                                       >  1:554835/1‑80 (MQ=255)                                   tataTTGTGGCGATCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTAc                                    <  1:554778/79‑1 (MQ=255)                                       ttGTGGCGATCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTACTGCaa                               <  2:554779/80‑1 (MQ=255)                                            gCGATCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTACTGCAACCgctg                         <  1:554780/81‑1 (MQ=255)                                            gCGATCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTACTGCAACCgctg                         <  1:554781/81‑1 (MQ=255)                                                tCAGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTACTGCAACCGCTGCTAc                     <  2:554782/81‑1 (MQ=255)                                                  aGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTACTGCAACCGCTGCTACa                    >  2:554819/1‑80 (MQ=255)                                                  aGTCTCGGCGTTGGCATGACTTCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTACTGCAACCGCTGCTACAt                   <  2:554783/81‑1 (MQ=255)                                                     ctcGGCGTTGGCATGACTTCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTACTGCAACCGCTGCTACATAGc                >  1:554827/1‑81 (MQ=255)                                                      tcGGCGTTGGCATGACTTCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTACTGCAACCGCTGCTACATAGCg               >  1:554831/1‑81 (MQ=255)                                                        ggCGTTGGCATGACTTCGACGCTCTATCACGATTTCTTTTCTAAGTTACCGGCCGTACTGCAACCGCTGCTACATAGCGGc             <  1:554784/81‑1 (MQ=255)                                                         gCGTTGGCATGACTTCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTACTGCAACCGCTGCTACATAGCGGCa            >  2:554844/1‑81 (MQ=255)                                                                 aTGACTTCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTACTGCAACCGCTGCTACATAGCGGCATTATGCTc    <  1:554786/81‑1 (MQ=255)                                                                 aTGACTTCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTACTGCAACCGCTGCTACATAGCGGCATTATGCTc    <  1:554785/81‑1 (MQ=255)                                                                   gACTTCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTACTGCAACCGCTGCTACATAGCGGCATTATGCTCg   >  2:554839/1‑80 (MQ=255)                                                                    aCTTCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTACTGCAACCGCTGCTACATAGCGGCATTATGCTCGc  <  2:554787/80‑1 (MQ=255)                                                                       |                                                                             GCGCTGTAACTACACCACCAACCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTCCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTACTGCAACCGCTGCTACATAGCGGCATTATGCTCGC  >  NC_000913/3032512‑3032658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑