Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,039,238	A→C	V169G (GTC→GGC)	xerD ←	site‑specific recombinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,039,238	0	A	C	100.0%	142.0 / NA	41	V169G (GTC→GGC)	xerD	site‑specific recombinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (25/16);  total (25/16)

TGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCA  >  NC_000913/3039160‑3039315                                                                               |                                                                              tGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGccc                                                                             >  1:556461/1‑81 (MQ=255)   ttCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGcccc                                                                            <  1:556418/80‑1 (MQ=255)     ccAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGt                                                                          <  1:556419/80‑1 (MQ=255)         aTAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCAc                                                                     <  2:556420/81‑1 (MQ=255)          tAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACg                                                                    >  2:556467/1‑81 (MQ=255)            ggTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGc                                                                   <  1:556421/80‑1 (MQ=255)             gTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGccc                                                                 >  2:556459/1‑81 (MQ=255)                 ccagccagTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGAc                                                             >  1:556478/1‑81 (MQ=255)                  cagccagTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGAc                                                             <  1:556422/80‑1 (MQ=255)                       agTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGc                                                       >  2:556479/1‑81 (MQ=255)                        gTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCt                                                      <  2:556423/81‑1 (MQ=255)                          aaaCCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGa                                                    >  2:556482/1‑81 (MQ=255)                              cGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATc                                                <  2:556424/81‑1 (MQ=255)                               gCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCa                                               >  1:556462/1‑81 (MQ=255)                                ccTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCAc                                              <  1:556425/81‑1 (MQ=255)                                ccTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCAc                                              >  1:556460/1‑81 (MQ=255)                                 ctctTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACt                                             <  2:556426/81‑1 (MQ=255)                                 ctctTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACt                                             >  2:556474/1‑81 (MQ=255)                                     tCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCAtt                                         >  2:556485/1‑81 (MQ=255)                                      cACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCAtt                                         <  1:556427/80‑1 (MQ=255)                                      cACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTg                                        >  2:556450/1‑81 (MQ=255)                                       aCCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGt                                       <  2:556428/81‑1 (MQ=255)                                          tAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAg                                    >  1:556481/1‑81 (MQ=255)                                           aaCGGCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGt                                   >  1:556470/1‑81 (MQ=255)                                              ggCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTcc                                 >  1:556472/1‑80 (MQ=255)                                              ggCACCAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTcc                                 >  1:556464/1‑80 (MQ=255)                                                  ccAGACGCTCTTTGTTGCCTTTACCAATGCCCCGTTCCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGAc                              >  1:556465/1‑79 (MQ=255)                                                       cGCTCTTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGtt                         >  2:556476/1‑79 (MQ=255)                                                          tctTTGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCa                       >  1:556471/1‑78 (MQ=255)                                                            tttGTTGCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCagag                    <  1:556429/79‑1 (MQ=255)                                                              tgttgCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGacac                >  1:556473/1‑81 (MQ=255)                                                               gttgCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACg               >  2:556469/1‑81 (MQ=255)                                                                  gCCTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAg            >  2:556504/1‑81 (MQ=255)                                                                    cTTTACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGcc          <  1:556430/81‑1 (MQ=255)                                                                     tttACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGccc         >  2:556483/1‑81 (MQ=255)                                                                       tACCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCg        <  2:556431/80‑1 (MQ=255)                                                                        aCCAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCgg       >  1:556475/1‑80 (MQ=255)                                                                         ccAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCgg       >  2:556477/1‑79 (MQ=255)                                                                         ccAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGt      <  1:556432/80‑1 (MQ=255)                                                                          cAATGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAg    <  2:556433/81‑1 (MQ=255)                                                                           aaTGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGc   <  1:556434/81‑1 (MQ=255)                                                                            aTGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCTGACCAGTTCAGAGACACGCAGCCCGGTAGCa  >  2:556490/1‑81 (MQ=255)                                                                            aTGCCCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCa  >  1:556501/1‑81 (MQ=255)                                                                               |                                                                              TGTTCCAGATAGGTTTCCAGCCAGTAAACCGCCTCTTCACCTAACGGCACCAGACGCTCTTTGTTGCCTTTACCAATGACCCGTACCACGCCCTGACGCAGGCTGATATCACTCATTGTCAGTCCGACCAGTTCAGAGACACGCAGCCCGGTAGCA  >  NC_000913/3039160‑3039315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑