Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	315,000	A→G	pseudogene (644/888 nt)	eaeH →	putative porin domain‑containing protein EaeH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	315,000	0	A	G	100.0%	98.7 / NA	29	pseudogene (644/888 nt)	eaeH	putative porin domain‑containing protein EaeH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/11);  total (18/11)

ATTTATCCCGTAGTCATACCCGCATTGGTGTTGGTGCGGAATACTGGCGCGATTATCTGAAACTGAGCGCCAATGGTTATATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTC  >  NC_000913/314922‑315077                                                                               |                                                                              aTTTATCCCGTAGTCATACCCGCATTGGTGTTGGTGCGGAATACTGGCGCGATTATCTGAAACTGAGCGCCAATGGTtgta                                                                             >  1:72432/1‑81 (MQ=255)   ttATCCCGTAGTCATACCCGCATTGGTGTTGGTGCGGAATACTGGCGCGATTATCTGAAACTGAGCGCCAATGGTTGTAtt                                                                           >  2:72444/1‑81 (MQ=255)     aTCCCGTAGTCATACCCGCATTGGTGTTGGTGCGGAATACTGGCGCGATTATCTGAAACTGAGCGCCAATGGTTGTATTc                                                                          <  2:72408/80‑1 (MQ=255)          gTAGTCATACCCGCATTGGTGTTGGTGCGGAATACTGGCGCGATTATCTGAAACTGAGCGCCAATGGTTGTATTCGGGCtt                                                                    >  2:72421/1‑81 (MQ=255)          gTAGTCATACCCGCATTGGTGTTGGTGCGGAATACTGGCGCGATTATCTGAAACTGAGCGCCAATGGTTGTATTCGGGCtt                                                                    <  1:72409/81‑1 (MQ=255)                aTACCCGCATTGGTGTTGGTGCGGAATACTGGCGCGATTATCTGAAACTGAGCGCCAATGGTTGTATTCGGGCTTctggct                                                              <  1:72410/81‑1 (MQ=255)                aTACCCGCATTGGTGTTGGTGCGGAATACTGGCGCGATTATCTGAAACTGAGCGCCAATGGTTGTATTCGGGCTTctggct                                                              >  2:72433/1‑81 (MQ=255)                  aCCCGCATTGGTGTTGGTGCGGAATACTGGCGCGATTATCTGAAACTGAGCGCCAATGGTTGTATTCGGGCTTctggctg                                                             >  2:72439/1‑80 (MQ=255)                   cccGCATTGGTGTTGGTGCGGAATACTGGCGCGATTATCTGAAACTGAGCGCCAATGGTTGTATTCGGGCTTctggctgg                                                            >  1:72440/1‑80 (MQ=255)                           ggtgttggtgCGGAATACTGGCGCGATTATCTGAAACTGAGCGCCAATGGTTGTATTCGGGCTTCTGGCTGGAAAAAATc                                                    <  1:72411/80‑1 (MQ=255)                                  gtgCGGAATACTGGCGCGATTATCTGAAACTGAGCGCCAATGGTTGTATTCGGGCTTCTGGCTGGAAAAAATCGCCGGata                                            >  2:72448/1‑81 (MQ=255)                                  gtgCGGAATACTGGCGCGATTATCTGAAACTGAGCGCCAATGGTTGTATTCGGGCTTCTGGCTGGAAAAAATCGCCGGat                                             <  1:72412/80‑1 (MQ=255)                                     cGGAATACTGGCGCGATTATCTGAAACTGAGCGCCAATGGTTGTATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATAtt                                          >  2:72447/1‑80 (MQ=255)                                       gAATACTGGCGCGATTATCTGAAACTGAGCGCCAATGGTTGTATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAg                                       >  1:72456/1‑81 (MQ=255)                                       gAATACTGGCGCGATTATCTGAAACTGAGCGCCAATGGTTGTATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAg                                       >  2:72435/1‑81 (MQ=255)                                        aaTACTGGCGCGATTATCTGAAACTGAGCGCCAATGGTTGTATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAg                                       >  2:72452/1‑80 (MQ=255)                                        aaTACTGGCGCGATTATCTGAAACTGAGCGCCAATGGTTGTATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAg                                       >  2:72438/1‑80 (MQ=255)                                             tGGCGCGATTATCTGAAACTGAGCGCCAATGGTTGTATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTAt                                 >  1:72455/1‑81 (MQ=255)                                                   gATTATCTGAAACTGAGCGCCAATGGTTGTATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGaa                           >  2:72437/1‑81 (MQ=255)                                                      tATCTGAAACTGAGCGCCAATGGTTGTATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGc                        >  1:72434/1‑81 (MQ=255)                                                       aTCTGAAACTGAGCGCCAATGGTTGTATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGc                        <  1:72413/80‑1 (MQ=255)                                                        tCTGAAACTGAGCGCCAATGGTTGTATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGcc                       <  2:72415/80‑1 (MQ=255)                                                        tCTGAAACTGAGCGCCAATGGTTGTATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGcc                       <  2:72414/80‑1 (MQ=255)                                                               cTGAGCGCCAATGGTTGTATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAAt               >  2:72443/1‑81 (MQ=255)                                                                  aGCGCCAATGGTTGTATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGc            >  1:72446/1‑81 (MQ=255)                                                                         aTGGTTGTATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGata     >  2:72460/1‑81 (MQ=255)                                                                          tGGTTGTATTAGGGCTTCTGGCTGGAAAAAAACGACGGATATTGAGGATTATCAGGAACGCCCGGAGAATGGCTGGGatat    <  2:72416/81‑1 (MQ=255)                                                                            gTTGTATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATAtt   <  2:72418/80‑1 (MQ=255)                                                                            gTTGTATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTc  <  2:72417/81‑1 (MQ=255)                                                                            gTTGTATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATAGTc  >  2:72463/1‑81 (MQ=255)                                                                               |                                                                              ATTTATCCCGTAGTCATACCCGCATTGGTGTTGGTGCGGAATACTGGCGCGATTATCTGAAACTGAGCGCCAATGGTTATATTCGGGCTTCTGGCTGGAAAAAATCGCCGGATATTGAGGATTATCAGGAACGCCCGGCGAATGGCTGGGATATTC  >  NC_000913/314922‑315077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑