Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,101,644	T→C	intergenic (‑21/+163)	yggN ← / ← yggL	putative EcfF/protein YggL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,101,644	0	T	C	100.0%	164.0 / NA	47	intergenic (‑21/+163)	yggN/yggL	putative EcfF/protein YggL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/20);  total (27/20)

GTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCT  >  NC_000913/3101567‑3101717                                                                              |                                                                          gTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAg                                                                        >  2:571385/1‑81 (MQ=255)  tGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAgg                                                                       >  2:571377/1‑81 (MQ=255)    aGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGcc                                                                     <  1:571318/81‑1 (MQ=255)        gTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCa                                                                 <  1:571319/81‑1 (MQ=255)          cATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCAtt                                                               >  2:571375/1‑81 (MQ=255)           aTTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCAtt                                                               <  2:682479/80‑1 (MQ=255)               ccGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCt                                                           <  1:571320/80‑1 (MQ=255)                cGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTgg                                                         >  1:571371/1‑81 (MQ=255)                 gTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGc                                                        >  2:571379/1‑81 (MQ=255)                 gTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGc                                                        <  2:571321/81‑1 (MQ=255)                   cACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGccc                                                      >  2:571380/1‑81 (MQ=255)                   cACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGccc                                                      >  1:571370/1‑81 (MQ=255)                   cACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGccc                                                      >  1:571378/1‑81 (MQ=255)                    aCTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGccc                                                      <  2:571322/80‑1 (MQ=255)                        aaaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAg                                                   <  2:571323/79‑1 (MQ=255)                         aaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGAtt                                                <  1:571324/81‑1 (MQ=255)                          aGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTg                                               <  2:571325/81‑1 (MQ=255)                               tgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATaa                                           >  1:571381/1‑80 (MQ=255)                                gccgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAAc                                          >  1:571394/1‑80 (MQ=255)                                 ccgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaa                                        <  1:571326/81‑1 (MQ=255)                                        cagcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgc                                 <  2:571327/81‑1 (MQ=255)                                          gcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGt                               >  1:571366/1‑81 (MQ=255)                                          gcaTTTTGCGCATCATAGTCTGCCCTCAAGAAAAAGCCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGt                               <  2:571328/81‑1 (MQ=255)                                           caTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGtt                              <  1:571329/81‑1 (MQ=255)                                            aTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGttt                             >  1:571397/1‑81 (MQ=255)                                            aTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGttt                             <  2:571330/81‑1 (MQ=255)                                             ttttGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGtttt                            <  2:571331/81‑1 (MQ=255)                                              tttGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTc                           >  1:571382/1‑81 (MQ=255)                                              tttGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTc                           >  2:571376/1‑81 (MQ=255)                                                tGCGCATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCa                         >  2:571391/1‑81 (MQ=255)                                                 gcgcATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAt                        >  1:571402/1‑81 (MQ=255)                                                  cgcATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATg                       >  1:571383/1‑81 (MQ=255)                                                   gcATCATAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGc                      >  1:571389/1‑81 (MQ=255)                                                     atcatAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCg                    >  2:571390/1‑81 (MQ=255)                                                       catAGTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGa                  >  2:571392/1‑81 (MQ=255)                                                           gccTTCCCTCAAGAAAAAACCATGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcg              >  1:571367/3‑81 (MQ=255)                                                           gTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcg              >  2:571384/1‑81 (MQ=255)                                                           gTCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcg              >  2:571406/1‑81 (MQ=255)                                                            tCTTCCCTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcgg             <  1:571332/81‑1 (MQ=255)                                                                cccTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcggcg           <  1:571333/79‑1 (MQ=255)                                                                cccTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGt          <  2:571334/80‑1 (MQ=255)                                                                cccTCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGt          >  2:571405/1‑80 (MQ=255)                                                                   tCAAGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTaaa       >  1:571457/1‑80 (MQ=255)                                                                     aaGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAac      <  2:571336/79‑1 (MQ=255)                                                                     aaGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAac      <  2:571335/79‑1 (MQ=255)                                                                      aGAAAAAACCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACAcc   >  1:571418/1‑81 (MQ=255)                                                                        aaaaaaCCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCt  >  2:571386/1‑80 (MQ=255)                                                                              |                                                                          GTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCT  >  NC_000913/3101567‑3101717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑