Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,102,267	C→A	D196Y (GAC→TAC)	trmI ←	tRNA m(7)G46 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,102,267	0	C	A	100.0%	121.0 / NA	34	D196Y (GAC→TAC)	trmI	tRNA m(7)G46 methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (8/26);  total (8/26)

TTGTTCAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCT  >  NC_000913/3102190‑3102344                                                                              |                                                                              ttgttCAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAAt                                                                            <  1:571485/81‑1 (MQ=255)      cAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATagaa                                                                        <  1:571486/80‑1 (MQ=255)        aaTTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATagaagaa                                                                     <  2:571487/81‑2 (MQ=255)            tCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATc                                                                  <  2:571488/80‑1 (MQ=255)              gTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATCACt                                                               <  2:571489/81‑1 (MQ=255)                      aCGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCat                                                       <  2:571491/81‑1 (MQ=255)                      aCGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCat                                                       <  1:571490/81‑1 (MQ=255)                      aCGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCat                                                       <  1:571492/81‑1 (MQ=255)                        gTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCatat                                                     >  2:571545/1‑81 (MQ=255)                        gTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCatat                                                     <  2:571493/81‑1 (MQ=255)                          gATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCATATGt                                                   >  1:571519/1‑81 (MQ=255)                           aTGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCATAtttt                                                  >  2:571542/1‑81 (MQ=255)                           aTGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCATATGtt                                                  >  2:571547/1‑81 (MQ=255)                           aTGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCATATGtt                                                  <  2:571494/81‑1 (MQ=255)                             gCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCATATGTTcc                                                >  2:571548/1‑81 (MQ=255)                               cGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCATATGTTCCGc                                              <  1:571495/81‑1 (MQ=255)                                      ggTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGt                                       <  2:571496/81‑1 (MQ=255)                                       gTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGtt                                      <  2:571497/81‑1 (MQ=255)                                       gTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGtt                                      <  2:571498/81‑1 (MQ=255)                                        tACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTc                                     <  2:571499/81‑1 (MQ=255)                                            tAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAg                                 <  2:571500/81‑1 (MQ=255)                                                 aTTGCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAgtcgg                             <  2:571501/80‑1 (MQ=255)                                                    gCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGc                         >  2:571541/1‑81 (MQ=255)                                                            aCAGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGa                 <  2:571502/81‑1 (MQ=255)                                                              aGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAAt               <  1:571504/81‑1 (MQ=255)                                                              aGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAAt               <  1:571505/81‑1 (MQ=255)                                                              aGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAAt               <  2:571503/81‑1 (MQ=255)                                                                   tttATAACCGTAAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGcc          >  1:571549/1‑81 (MQ=255)                                                                    ttATAACCGTAAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGcc          <  2:571506/80‑1 (MQ=255)                                                                       taACCGTAAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGccccc       <  1:571507/80‑1 (MQ=255)                                                                        aaCCGTAAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGccccc       >  1:571543/1‑79 (MQ=255)                                                                         aCCGTAAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAg    <  1:571508/81‑1 (MQ=255)                                                                           cGTAAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCt  <  1:571510/81‑1 (MQ=255)                                                                           cGTAAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCt  <  1:571509/81‑1 (MQ=255)                                                                              |                                                                              TTGTTCAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCT  >  NC_000913/3102190‑3102344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑