Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,102,776	C→T	R26H (CGC→CAC)	trmI ←	tRNA m(7)G46 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,102,776	0	C	T	100.0%	136.5 / NA	38	R26H (CGC→CAC)	trmI	tRNA m(7)G46 methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/21);  total (17/21)

CTTCGCTGAACTCAACGCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGCGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTGAAATGACGTCGTTTTTC  >  NC_000913/3102699‑3102850                                                                              |                                                                           cTTCGCTGAACTCAACGCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTggtggc                                                                         >  2:571647/1‑81 (MQ=255)   tCGCTGAACTCAACGCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGgtggcg                                                                        >  2:571645/1‑80 (MQ=255)    cGCTGAACTCAACGCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCtggtgg                                                                          <  2:571592/77‑1 (MQ=255)    cGCTGAACTCAACGCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGca                                                                      >  1:571649/1‑81 (MQ=255)        gAACTCAACGCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACaa                                                                   >  2:571638/1‑80 (MQ=255)              aaCGCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACg                                                            >  1:571635/1‑81 (MQ=255)                cGCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGa                                                          >  2:571644/1‑81 (MQ=255)                 gCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGAt                                                         <  2:571596/81‑1 (MQ=255)                 gCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGAt                                                         <  2:571594/81‑1 (MQ=255)                 gCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTAGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGAt                                                         <  1:571595/81‑1 (MQ=255)                 gCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTAGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGAt                                                         <  1:571593/81‑1 (MQ=255)                  cccATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATa                                                        >  1:571634/1‑81 (MQ=255)                    cATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACg                                                      >  1:571643/1‑81 (MQ=255)                    cATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACg                                                      <  1:571597/81‑1 (MQ=255)                      tCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGgc                                                    <  1:571600/81‑1 (MQ=255)                      tCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGgc                                                    <  2:571599/81‑1 (MQ=255)                      tCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGgc                                                    <  2:571598/81‑1 (MQ=255)                           ggCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGt                                               <  1:571601/81‑1 (MQ=255)                            gCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTGTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTg                                              <  1:571602/81‑1 (MQ=255)                               agtagtTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGggc                                           <  1:571604/81‑1 (MQ=255)                               agtagtTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGggc                                           <  2:571603/81‑1 (MQ=255)                                gtagtTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGggcg                                          <  2:571605/81‑1 (MQ=255)                                       tCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGttt                                   >  2:571637/1‑81 (MQ=255)                                        ccAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGttt                                   <  2:571606/80‑1 (MQ=255)                                         cAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGtttt                                  <  1:571607/80‑1 (MQ=255)                                         cAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGtttt                                  >  1:571655/1‑80 (MQ=255)                                         cAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGtttt                                  >  1:571651/1‑80 (MQ=255)                                                  ttCCTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTc                        >  1:571681/1‑81 (MQ=255)                                                    ccTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTcc                       <  2:571608/80‑1 (MQ=255)                                                    ccTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTcc                       <  2:571609/80‑1 (MQ=255)                                                     cTGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCgg                     >  2:571659/1‑81 (MQ=255)                                                      tGGCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGt                    >  1:571652/1‑81 (MQ=255)                                                        gCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTg                   >  2:571662/1‑80 (MQ=255)                                                        gCCTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTg                   >  1:571653/1‑80 (MQ=255)                                                         ccTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTGa                  >  2:571650/1‑80 (MQ=255)                                                          cTTTGGTCAGTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTGaaa                >  1:571660/1‑81 (MQ=255)                                                                   gTCGCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTGAAATGAcgtcgt       <  2:571610/81‑1 (MQ=255)                                                                      gCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTGAAATGACGTCGtttt    >  2:571657/1‑81 (MQ=255)                                                                      gCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTGAAATGACGTCGttt     <  1:571611/80‑1 (MQ=255)                                                                      gCCCCTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTGAAATGACGTCGttt     <  1:571612/80‑1 (MQ=255)                                                                        cccTGGTGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTGAAATGACGTCGTTTTTc  <  2:571613/81‑1 (MQ=255)                                                                              |                                                                           CTTCGCTGAACTCAACGCCCATCACCGGCCAGTAGTTTTCCAGCGCATGTTCCTGGCCTTTGGTCAGTCGCCCCTGGCGGCGCACAAAACTACGGATACGGCGCAGTGGGCGGCCGTTTTCATCAAATTCCGGTGAAATGACGTCGTTTTTC  >  NC_000913/3102699‑3102850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑