Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,107,146	T→C	Q670Q (CAA→CAG)	speC ←	ornithine decarboxylase, biosynthetic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,107,146	0	T	C	100.0%	104.0 / NA	31	Q670Q (CAA→CAG)	speC	ornithine decarboxylase, biosynthetic
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/9);  total (22/9)

CTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCG  >  NC_000913/3107072‑3107222                                                                           |                                                                             cTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGCTGAAcc                                                                        <  2:572601/81‑1 (MQ=255)   ataAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGCTGAACCGc                                                                      >  2:572658/1‑81 (MQ=255)      aaCACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGCTGAACCGccc                                                                    >  2:572644/1‑80 (MQ=255)        cacCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGCTGAACCGccccac                                                                 >  2:572643/1‑81 (MQ=255)              gCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGCTGAACCGCCCCACCCCAGa                                                           >  1:572652/1‑81 (MQ=255)               cAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGCTGAACCGCCCCACCCCAGAc                                                          >  1:572654/1‑81 (MQ=255)                   tCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGCTGAACCGCCCCACCCCAGACTTcc                                                      >  1:572655/1‑81 (MQ=255)                    ccGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGCTGAACCGCCCCACCCCAGACTTccc                                                     >  2:572666/1‑81 (MQ=255)                       gCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGCTGAACCGCCCCACCCCAGACTTCCCCgg                                                  >  2:572640/1‑81 (MQ=255)                         gAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGCTGAACCGCCCCACCCCAGACTTCCCCggg                                                 >  2:572646/1‑80 (MQ=255)                         gAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGCTGAACCGCCCCACCCCAGACTTCCCCggg                                                 >  2:572634/1‑80 (MQ=255)                           aaaTCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGCTGAACCGCCCCACCCCAGACTTCCCCGGGTAc                                              <  2:572602/81‑1 (MQ=255)                                 cGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGCTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGc                                         >  1:572631/1‑80 (MQ=255)                                  ggCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGCTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCa                                        >  1:572642/1‑80 (MQ=255)                                       caaATTCACCCCTTCTTCCAGTGCAAGGAAATAACGCTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCAc                                  <  2:572603/81‑1 (MQ=255)                                         aaTTCACCCCTTCTTCCAGTGCAAGGAAATAACGCTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGc                                >  1:572650/1‑81 (MQ=255)                                          aTTCACCCCTTCTTCCAGTGCAAGGAAATAACGCTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGcc                               <  1:572604/81‑1 (MQ=255)                                             cACCCCTTCTTCCAGTGCAAGGAAATAACGCTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAg                             <  2:572605/80‑1 (MQ=255)                                                   ttcttcCAGTGCAAGGAAATAACGCTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGAt                       >  2:572665/1‑80 (MQ=255)                                                     cttcCAGTGCAAGGAAATAACGCTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAg                    >  1:572663/1‑81 (MQ=255)                                                        ccAGTGCAAGGAAATAACGCTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGc                  >  2:572662/1‑80 (MQ=255)                                                        ccAGTGCAAGGAAATAACGCTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGc                  >  2:572668/1‑80 (MQ=255)                                                         cAGTGCAAGGAAATAACGCTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCaa                <  2:572606/81‑1 (MQ=255)                                                          aGTGCAAGGAAATAACGCTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAAc               >  2:572664/1‑81 (MQ=255)                                                              cAAGGAAATAACGCTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGccc           >  1:572669/1‑81 (MQ=255)                                                                   aaaTAACGCTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCt      <  1:572607/81‑1 (MQ=255)                                                                    aaTAACGCTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTg     >  1:572649/1‑81 (MQ=255)                                                                    aaTAACGCTGAACCGCCCCAACCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTg     <  2:572608/81‑1 (MQ=255)                                                                      tAACGCTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGcc   >  2:572648/1‑81 (MQ=255)                                                                        aCGCTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCg  >  2:572657/1‑80 (MQ=255)                                                                        aCGCTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCg  <  2:572609/80‑1 (MQ=255)                                                                           |                                                                             CTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATAACGTTGAACCGCCCCACCCCAGACTTCCCCGGGTACCACGCAAAGCACGCCAGGTGGATAAGGCAACGCCCCTTCTGCCG  >  NC_000913/3107072‑3107222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑