Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,123,304	C→T	A168T (GCC→ACC)	glcB ←	malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,123,304	0	C	T	100.0%	145.2 / NA	41	A168T (GCC→ACC)	glcB	malate synthase G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/23);  total (18/23)

CACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTAT  >  NC_000913/3123227‑3123380                                                                              |                                                                             caccTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGAt                                                                           <  2:576395/81‑1 (MQ=255)        aaCGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGc                                                                    >  2:576458/1‑81 (MQ=255)           gCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCa                                                                 >  2:576473/1‑81 (MQ=255)             caccacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCAcc                                                               >  1:576462/1‑81 (MQ=255)              accacATCCTGATAGCTGCCGTTTTCCAGCAGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACcg                                                              <  2:576396/81‑1 (MQ=255)                 acaTCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGt                                                           <  1:576397/81‑1 (MQ=255)                  caTCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGtt                                                          <  1:576398/81‑1 (MQ=255)                      cTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCgg                                                      <  2:576399/81‑1 (MQ=255)                           aGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCg                                                  >  2:576447/1‑80 (MQ=255)                            gCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGt                                                 <  2:576402/80‑1 (MQ=255)                            gCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGt                                                 <  2:576401/80‑1 (MQ=255)                            gCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTa                                                <  2:576400/81‑1 (MQ=255)                             cTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAg                                               <  2:576403/81‑1 (MQ=255)                              tGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGc                                              >  1:576468/1‑81 (MQ=255)                                ccGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCg                                            <  2:576404/81‑1 (MQ=255)                                  gTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCt                                          >  2:576478/1‑81 (MQ=255)                                  gTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCt                                          <  1:576405/81‑1 (MQ=255)                                      tCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGAc                                       >  2:576455/1‑80 (MQ=255)                                        cAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAt                                    >  1:576489/1‑81 (MQ=255)                                        cAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCAt                                    >  1:576461/1‑81 (MQ=255)                                               agagATTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCt                             >  1:576475/1‑81 (MQ=255)                                                gagaTTCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCtt                            <  1:576406/81‑1 (MQ=255)                                                     tCATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGc                       >  1:576465/1‑81 (MQ=255)                                                      cATCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGc                       <  1:576408/80‑1 (MQ=255)                                                       aTCGAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCgg                     >  2:576488/1‑81 (MQ=255)                                                          gAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGgat                  <  1:576410/81‑1 (MQ=255)                                                          gAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGgat                  >  1:576471/1‑81 (MQ=255)                                                          gAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGgat                  <  1:576411/81‑1 (MQ=255)                                                          gAGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGgat                  <  2:576409/81‑1 (MQ=255)                                                           aGGAAACGCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGgatg                 <  1:576412/81‑1 (MQ=255)                                                                  gCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGc          >  2:576479/1‑81 (MQ=255)                                                                  gCCGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGc          >  2:576480/1‑81 (MQ=255)                                                                   ccGAACCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCt         >  2:576459/1‑81 (MQ=255)                                                                       aCCCAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCg     <  2:576413/81‑1 (MQ=255)                                                                        cccAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGt    <  1:576415/81‑1 (MQ=255)                                                                        cccAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGt    <  2:576414/81‑1 (MQ=255)                                                                         ccAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGta   <  2:576416/81‑1 (MQ=255)                                                                          cAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGtat  <  1:576418/81‑1 (MQ=255)                                                                          cAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGtat  <  1:576417/81‑1 (MQ=255)                                                                          cAGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGta   >  1:576500/1‑80 (MQ=255)                                                                           aGGTGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTACCTGCGGGATGATGTCGCTGCCGtat  >  2:887278/1‑80 (MQ=255)                                                                              |                                                                             CACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTAT  >  NC_000913/3123227‑3123380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑