Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,139,574	A→T	E283V (GAA→GTA)	yghU →	disulfide reductase/organic hydroperoxide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,139,574	0	A	T	100.0%	135.5 / NA	38	E283V (GAA→GTA)	yghU	disulfide reductase/organic hydroperoxide reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (22/16);  total (22/16)

GTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCAT  >  NC_000913/3139498‑3139649                                                                             |                                                                            gTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGa                                                                          <  1:580407/80‑1 (MQ=255)     aCCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGc                                                                     >  2:580443/1‑81 (MQ=255)       cGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGt                                                                   >  2:580483/1‑81 (MQ=255)          aCCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAg                                                                <  1:580409/81‑1 (MQ=255)           ccAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAgg                                                               <  2:580410/81‑1 (MQ=255)             aaCGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAgggg                                                             >  1:580459/1‑81 (MQ=255)             aaCGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAgggg                                                             >  2:580451/1‑81 (MQ=255)              aCGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAgggg                                                             >  2:580455/1‑80 (MQ=255)               cGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTa                                                           <  2:580412/81‑1 (MQ=255)               cGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTa                                                           <  2:580411/81‑1 (MQ=255)                ggACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGt                                                            >  2:580464/1‑79 (MQ=255)                ggACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGt                                                            >  1:580482/1‑79 (MQ=255)                    cGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGt                                                      >  2:580476/1‑81 (MQ=255)                    cGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGt                                                      >  1:580477/1‑81 (MQ=255)                        gAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGgt                                                  >  2:580463/1‑81 (MQ=255)                         aaTGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGgt                                                  <  2:580413/80‑1 (MQ=255)                         aaTGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTCGgt                                                  <  2:580414/80‑1 (MQ=255)                          aTGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGgtgt                                                <  1:580416/81‑1 (MQ=255)                          aTGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGgtgt                                                <  2:580415/81‑1 (MQ=255)                          aTGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGgtgt                                                >  1:580475/1‑81 (MQ=255)                            gAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTtc                                              >  1:580481/1‑81 (MQ=255)                                aGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTtcgtcg                                          >  1:580470/1‑81 (MQ=255)                                 gTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTtcgtcg                                          >  1:580479/1‑80 (MQ=255)                                  ttGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTtcgtcg                                          >  1:580467/1‑79 (MQ=255)                                     cATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCgcagc                                      <  1:580417/80‑1 (MQ=255)                                      aTGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCgcagc                                      >  1:580474/1‑79 (MQ=255)                                          gcgaCATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCa                                <  2:580418/81‑1 (MQ=255)                                              cATGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCa                            <  2:580419/81‑1 (MQ=255)                                               aTGACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAg                           >  1:580469/1‑81 (MQ=255)                                                 gACGCCAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGc                         >  2:580480/1‑81 (MQ=255)                                                      cAGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGAt                    <  1:580420/81‑1 (MQ=255)                                                       aGTGATTTCGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATa                   <  2:580421/81‑1 (MQ=255)                                                               cGAGACGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGtt            >  1:580489/1‑80 (MQ=255)                                                                gagaCGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCa          >  2:580473/1‑81 (MQ=255)                                                                 agaCGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAc         <  1:580422/81‑1 (MQ=255)                                                                    cGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgcc      <  1:580423/81‑1 (MQ=255)                                                                    cGAATACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgcc      >  1:580488/1‑81 (MQ=255)                                                                        tACGGTAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCAt  >  1:580471/1‑81 (MQ=255)                                                                             |                                                                            GTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCAT  >  NC_000913/3139498‑3139649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑